Sequences from confirmation amplicon


Confirmation sequences for line 014E07 and hit At1g21670 are shown.
Line ID 014E07
Locus At1g21670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7611094
Primers used for PCR 15lj - 8474, show primer details
   
Sequence Name 49-K113947-0022-014-E07C-15lj
GenBank Accession LN506915 [GenBank]
Sequencing primer gene specific primer, 15lj: 5'-CAAAAGAACTCAGATTCAACCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K113947-0022-014-E07C-15lj
GGACTCCTAGCTTAACTACGACGTCGTTCACAGCGATAACGTTGGATCTAGAATTCAGGT
TCCGTTATTTTCCGGCGAGGAACAACAGAGTGGTATGAATGTAAATTCCATGAAAGACAC
GCCTGTTTTAACAAACGGCTATCTTGTCCACGTGTCGACACATGAGAATCCGGGTAAGCC
CATGGCGAGTTGGGCCGCTGTTTACTCGACTGAGTTACGAACTAAGTCGACTCGGCGGCT
TACACCACTGGGTATCGCTGACTTTTCTCCGGCTGTGTCTCCGTCTGGGAAGTGGACTGC
CGTGGCTTCGTTTGGAGAGAAAGGCTGGACTTGGAGCATGGTAGAGAAAGAGATTAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTACGCA
   
Sequence Name 21-K113946-0022-014-E07C-8409
GenBank Accession LN495749 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K113946-0022-014-E07C-8409
TTGAATATATCCTAATCTCTTTCTCTACCATGCTCCAAGTCCAGCCTTTCTCTCCAAACG
AAGCCACGGCAGTCCACTTCCCAGACGGAGACACAGCCGGAGAAAAGTCAGCGATACCCA
GTGGTGTAAGCCGCCGAGTCGACTTAGTTCGTAACTCAGTCGAGTAAACAGCGGCCCAAC
TCGCCATGGGCTTACCCGGATTCTCATGTGTCGACACGTGGACAAGATAGCCGTTTGTTA
AAACAGGCGTGTCTTTCATGGAATTTACATTCATACCACTCTGTTGTTCCTCGCCGGAAA
ATAACGGAACCTGAATTCTAGATCCAACGTTATCGCTGTGAACGACGTCGTAGTTTAAGC
TAGGAGTTCCGGCTCTCTCAGTGACGTAGATGAGATGCACGTCTTGTGGTTGAATCTANT
TTCTTTTG

Last Updated on 06.12.2018 16:11