Sequences from confirmation amplicon


Confirmation sequences for line 015F09 and hit At1g74850 are shown.
Line ID 015F09
Locus At1g74850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28121624
Primers used for PCR 16lj - 8474, show primer details
   
Sequence Name 62-K114067-0022-015-F09C-16lj
GenBank Accession LN510811 [GenBank]
Sequencing primer gene specific primer, 16lj: 5'-ATCATGGAACAAAGTTACCACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114067-0022-015-F09C-16lj
CTCATCAAATGTTATATGTAGCAGCATCAGGATCCGTATTGCTTGATTTCATCTCAAGAA
AAAGCTGCCTCACATCATCATACCTCCCACTCTGTCCAAACAAATTCAGCAAAACACTGT
ATGTATTTGCGTTCGGCGTACACCCTGCAGCTTGCATCTGATGGAAGACCCCCATTGCCT
CCTTAATAGACCCGGATTTCGCATATGCCTCTAACAACACATTATAAGAAGTGATATCCG
GCAAACTACCGCCGGAGGCCATCTCGCCTAAAAGATCACATACTTTCTCCAGCCGGCGTA
ACTTCCCAAATGTCTCAACTAGATGACTATATGTGGTTAAATCCGGAACAATCCCACCAT
CATTCATAGTCCTAAAAACCATTTCAGCCTCATCACCTAAACCTCTAATAGCACAAGCAC
TAAGCAGTGTATTATAAGTCACGATATCAGGTTGTATCCCCTCATGTCTCATCTCTGCAA
ACAAACCCAATAAACCCTCCCAATCTAAACCACCTGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAAATGTCCGCA
   
Sequence Name 37-K114066-0022-015-F09C-8409
GenBank Accession LN502694 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K114066-0022-015-F09C-8409
GGAGGTTATTGGGTTTGTTTGCAGAGATGAGACTGAGGGGATACAACCTGATATCGTGAC
TTATAATACACTGCTTAGTGCTTGTGCTATTAGAGGTTTAGGTGATGAGGCTGAAATGGT
TTTTAGGACTATGAATGATGGTGGGATTGTTCCGGATTTAACCACATATAGTCATCTAGT
TGAGACATTTGGGAAGTTACGCCGGCTGGAGAAAGTATGTGATCTTTTAGGCGAGATGGC
CTCCGGCGGTAGTTTGCCGGATATCACTTCTTATAATGTGTTGTTAGAGGCATATGCGAA
ATCCGGGTCTATTAAGGAGGCAATGGGGGTCTTCCATCAGATGCAAGCTGCAGGGTGTAC
GCCGAACGCAAATACATACAGTGTTTTGCTGAATTTGTTTGGACAGAGTGGGAGGTATGA
TGATGTGAGGCAGCTTTTTCTTGAGATGAAATCAAGCAATACGGATCCTGATGCTGCTAC
ATATAACATTTTGATTGAGGTTTTTGGGGAAGGGGGGTATTTTAAGGAGGTGGTAACTTT
GTTCCATGA

Last Updated on 06.12.2018 16:11