Sequences from confirmation amplicon


Confirmation sequences for line 016A11 and hit At2g23540 are shown.
Line ID 016A11
Locus At2g23540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10025575
Primers used for PCR 01kq - 8474, show primer details
   
Sequence Name 53-K113756-0022-016-A11C-01kq
GenBank Accession LN508216 [GenBank]
Sequencing primer gene specific primer, 01kq: 5'-TTCTTCAACACTACACGGAAACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113756-0022-016-A11C-01kq
GCAAGATTCATAGCCAAGAAATCGATATTCTCAATCACTATAGGAGCAAACGATTTCCTC
AACAATTATCTATTCCCACTACTCTCGGTAGGAACACGGTTCACTCAAACTCCCGATGAT
TTTATTGGCGACATGCTTGAACATCTACGCGACCAACTAACCGTAAGCAACAAAATATTG
ATCTCTATCCATCAAAATAAAGTTAGAGAATTTTTGTTTAGTTCATTTGTTATCATATGC
ATGTTATGGTTGAAATACAGAGGCTGTATCAATTGGATGCGAGGAAGTTTGTGATCGGGA
ACGTTGGACCGATTGGATGCATACCGTACCAGAAAACAATCAATCAATTAGACGAAAACG
AGTGTGTGGATTTGGCTAATAAACCAATGAGTATGATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 15-K113755-0022-016-A11C-8409
GenBank Accession LN493054 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K113755-0022-016-A11C-8409
TCATTGGTTTATAGCCAATCCACACACTCGTTTTCGTCTACTTTATAGATTGTTTTCTGG
TACGGTATGCATCCAATCGGTCCAACGTTCCCGATCACAAACTTCCTCGCATCCAATTGA
TACAGCCTCTGTATTTCAACCATAACATGCATATGATAACAAATGAACTAAACAAAAATT
CTCTAACTTTATTTTGATGGATAGAGATCAATATTTTGTTGCTTACGGTTAGTTGGTCGC
GTAGATGTTCAAGCATGTCGCCAATAAAATCATCGGGAGTTTGAGTGAACCGTGTTCCTA
CCGAGAGTAGTGGGAATAGATAATTGTTGAGGAAATCATTTGCTCCTATAGTGATTGAGA
ATATCGATTTCTTGGCTATGTAATCTTTTGCTTTCTCTTTTCCAAGTAGATCATCAAACT
GTTTCCGTGTATGTTTGAAGAAA

Last Updated on 06.12.2018 16:11