Sequences from confirmation amplicon


Confirmation sequences for line 016F01 and hit At5g65687 are shown.
Line ID 016F01
Locus At5g65687 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26270443
Primers used for PCR PA07 - 8474, show primer details
   
Sequence Name 09-K037015-022-016-F01C-PA07
GenBank Accession LN490087 [GenBank]
Sequencing primer gene specific primer, PA07: 5'-GCAGAAGCTTCACAAATTAAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K037015-022-016-F01C-PA07
CCTCTCTTCTTTGTTACTGTCTATGTAAAAACAACTATATATACTAATATACTTCTTTTT
GGGGCAGATCCAAGAACCTGGTTGTCCTATTTGGAAAAGACTTAAAGGCTCTTTTCAGCG
AGAAAGTATTTATTGTAAATGTTCTTGGTTACATCACCTACAACTTTGTGATTGGAGCTT
ACTCATATTGGGGACCAAAAGCCGGTTTTGGTATCTACAAAATGAAAAACGCGGACATGA
TTTTTGGAGGACTTACAATCATATGCGGAATAATTGGAACATTAGGTGGAAGCTATGTGC
TTGATCGCATAAACGCTACTCTTTCAAACACCTTCAAGGTAATAAAGAACTCACTAAACA
CCTCTTAGTTACTATGAGTGCTCCGTTTTTTCTGATTACCTCTTAATTGATCTTGTACAG
TTACTAGCTGCATCAACTTTACTCGGCGCAGCGTTTTGTTTCACTGCTTTCTTGATGAAG
AATATGTATGCCTTCATCGCTTTATTCGCAGTGGGAGAAATTCTAATCTTTGCACCACAG
GTAACATAATCAAATCTCAAGTATTTTGCCAAAAAGCTAATCTCTGTTTCTTTTTTTTCG
ACTTGTTTGATCCGAATGTCATTTTGCTTTCTTGTGCAGGCTCCGGTTAACTTTGTGTGT
CTCCATTGCGTGAGACCAAACTTGAGACCATTGTCAATGGCTTCTTCAACTGTTTTGATC
CATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
   
Sequence Name 10-K037015-022-016-F01C-8409
GenBank Accession LN490562 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K037015-022-016-F01C-8409
CATTTACAATGGATCAAACAGTTGAAGAAGCCATTGACAATGGTCTCAAGTTTGGTCTCA
CGCAATGGAGACACACAAAGTTAACCGGAGCCTGCACAAGAAAGCAAAATGACATTCGGA
TCAAACAAGTCGAAAAAAAAGAAACAGAGATTAGCTTTTTGGCAAAATACTTGAGATTTG
ATTATGTTACCTGTGGTGCAAAGATTAGAATTTCTCCCACTGCGAATAAAGCGATGAAGG
CATACATATTCTTCATCAAGAAAGCAGTGAAACAAAACGCTGCGCCGAGTAAAGTTGATG
CAGCTAGTAACTGTACAAGATCAATTAAGAGGTAATCAGAAAAAACGGAGCACTCATAGT
AACTAAGAGGTGTTTAGTGAGTTCTTTATTACCTTGAAGGTGTTTGAAAGAGTAGCGTTT
ATGCGATCAAGCACATAGCTTCCACCTAATGTTCCAATTATTCCGCATATGATTGTAAGT
CCTCCAAAAATCATGTCCGCGTTTTTCATTTTGTAGATACCAAAACCGGCTTTTGGTCCC
CAATATGAGTAAGCTCCAATCACAAAGTTGTAGGTGATGTAACCAAGAACATTTACAATA
AATACTTTCTCGCTGAAAAGAGCCTTTAAGTCTTTTCCAAATAGGACAACCAGGTTCTTG
GATCTGCCCCAAAAAGAAGTATATTAGTATATATAGTTGTCTTTTACATAGACAGTAACA
AAGAAGAGAGGATAGCGAAACTCACTTGGGGGTTTTGGTTTTAATT

Last Updated on 06.12.2018 16:11