Sequences from confirmation amplicon


Confirmation sequences for line 016F08 and hit At3g05400 are shown.
Line ID 016F08
Locus At3g05400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1550800
Primers used for PCR 06bz - 8474, show primer details
   
Sequence Name 20-K109234-0022-016-F08C-06bz
GenBank Accession LN495247 [GenBank]
Sequencing primer gene specific primer, 06bz: 5'-CAGGAAATAGCTTTACCACGTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K109234-0022-016-F08C-06bz
CTCATTCACCAAATCGCCCCGGNTCAAGCCAAATAATATCCTGTGTGCCAAAAAAGAAAT
TTAAAATAAGAATTATATACTACAAAAAAACATAGACTATATTACTAAAATGGTGATAAA
ATATACAATTTTGTTAAACTTGCAAAAATAAAGAAGAATATAAGTTATGGAGGGAACCTT
GGCTAGTGAAATTGCAAGCCAACCAGTTATGCAGAATAAATCAGCCACCCACAACGTCTA
AGAAATATTGTAAGACAAAAAAAAATGAGGGTGTAGAAATTTACGATATAAATTTTTGGG
CTTACGACAAAATAAAAGAAAGATAGGGGTGATTTTGAGAATAATTGATACTTACCATTT
TATGACCGAAGGCACTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
   
Sequence Name 04-K109233-0022-016-F08C-8409
GenBank Accession LN487956 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K109233-0022-016-F08C-8409
TCGGACATGAGCCATTTACAATTGAATATATCCTGCAAGTGCCTTCGGTCATAAAATGGT
AAGTATCAATTATTCTCAAAATCACCCCTATCTTTCTTTTATTTTGTCGTAAGCCCAAAA
ATTTATATCGTAAATTTCTACACCCTCATTTTTTTTTGTCTTACAATATTTCTTAGACGT
TGTGGGTGGCTGATTTATTCTGCATAACTGGTTGGCTTGCAATTTCACTAGCCAAGGTTC
CCTCCATAACTTATATTCTTCTTTATTTTTGCAAGTTTAACAAAATTGTATATTTTATCA
CCATTTTAGTAATATAGTCTATGTTTTTTTGTAGTATATAATTCTTATTTTAAATTTCTT
TTTTGGCACACAGGATATTATTTGGCTTGACATGGGGCGATTTTTGGTGGGAATTGGAGT
TGGTCTCATTAGCTACGTGGTAAAGCTATTTCCTG

Last Updated on 06.12.2018 16:11