Sequences from confirmation amplicon


Confirmation sequences for line 017D09 and hit At2g31020 are shown.
Line ID 017D09
Locus At2g31020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13202427
Primers used for PCR 03vo - 8474, show primer details
   
Sequence Name 38-K117953-0022-017-D09C-03vo
GenBank Accession LN503140 [GenBank]
Sequencing primer gene specific primer, 03vo: 5'-GAGAAGCCATTGCTTTTGTTTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K117953-0022-017-D09C-03vo
ACCTCGTGATTTGTTCGCAGTCTTGAATGGCTGACTCACTCACTCCTTCCTCAACCAATC
GCAGCCTAAGCTTCTCTATCGAGATGTCTAAGTTGTTGGTCGGTGCCATCAACTCGCAGT
TTGACATTCTTGGAAACATATCTTTGACAGCTTGTAAAGCCTCTATCCAAGCTTCTCTGT
CCTCTCTTGTCTCTGCACGTAGGTGAAGCCTCTTGGTGCCGGTGAATATTGAAAATCTCT
TATCATCTGATCTACTCTCCCGAATCGATGAAACCTATTATATATACCACCGATCGAGTA
GATGATGATCAAATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCACACAGTTATTAT
   
Sequence Name 14-K117952-0022-017-D09C-8409
GenBank Accession LN492701 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117952-0022-017-D09C-8409
TCGGATGAGCATTTCATTGAAGCCATTTGATCATCATCTACTCGATCGGTGGTATATATA
ATAGGTTTCATCGATTCGGGAGAGTAGATCAGATGATAAGAGATTTTCAATATTCACCGG
CACCAAGAGGCTTCACCTACGTGCAGAGACAAGAGAGGACAGAGAAGCTTGGATAGAGGC
TTTACAAGCTGTCAAAGATATGTTTCCAAGAATGTCAAACTGCGAGTTGATGGCACCGAC
CAACAACTTAGACATCTCGATAGAGAAGCTTAGGCTGCGATTGGTTGAGGAAGGAGTGAG
TGAGTCAGCCATTCAAGACTGCGAACAAATCACGAGGTCTGAGTTCTCGGCAATTCAGAG
CCAGCTTTTGCTTCTTAAACAAAAGCAATGGGCTTCTC

Last Updated on 06.12.2018 16:11