Sequences from confirmation amplicon


Confirmation sequences for line 018D02 and hit At1g77310 are shown.
Line ID 018D02
Locus At1g77310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29053261
Primers used for PCR 01dl - 8474, show primer details
   
Sequence Name 17-K109936-0022-018-D02C-01dl
GenBank Accession LN493893 [GenBank]
Sequencing primer gene specific primer, 01dl: 5'-GCGTGAACTCTCCTAATTAAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K109936-0022-018-D02C-01dl
ATCTTTCTACGTCTCAAGATTAGAGAAGCATCACTCTTGGATGTAGAAGATGAAAGCCCC
AAATACATGCTTACTTCAAATTCCAAACTAACTAAAATGAGGAATCTAATAAACTTAAGA
TAGCTGGAAAGTACTTACAGATATTTGAATAGATGTTTTGCTTTCCGTTGCAATGTCCAG
GTCAGGCATGCCATTGTTAACTTGCTGACGAACTACTGCTGATTTTGCCGAAACATTTGG
CCGAGATTTTCCTGGAGGGGAATTAGATTGTTCATGAACACTTTTGCTGCTGCACTTCGG
ATGAAGGGGGCCAGAAGATCCAGCTGCCTTTGACTTCGGACTCGTGGCATTCATTGGCTG
GTGATTCGCTTTATCAGTATGCTTCACATTTTCCAAAACTAAGGAATCATTGCCATTTTG
CGCTTTCAAAGGGGAAAACGAATCTTGCACCGTCTTTGAATCATTGGAAATTTTCTTCGA
AGAGGGCCCAGGAGAAGCAGATTGATCTACTTGCTTCGACAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 01-K109935-0022-018-D02C-8409
GenBank Accession LN486617 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K109935-0022-018-D02C-8409
ATTGAATATATCCTGTCGAAGCAAGTAGATCAATCTGCTTCTCCTGGGCCCTCTTCGAAG
AAAATTTCCAATGATTCAAAGACGGTGCAAGATTCGTTTTCCCCTTTGAAAGCGCAAAAT
GGCAATGATTCCTTAGTTTTGGAAAATGTGAAGCATACTGATAAAGCGAATCACCAGCCA
ATGAATGCCACGAGTCCGAAGTCAAAGGCAGCTGGATCTTCTGGCCCCCTTCATCCGAAG
TGCAGCAGCAAAAGTGTTCATGAACAATCTAATTCCCCTCCAGGAAAATCTCGGCCAAAT
GTTTCGGCAAAATCAGCAGTAGTTCGTCAGCAAGTTAACAATGGCATGCCTGACCTGGAC
ATTGCAACGGAAAGCAAAACATCTATTCAAATATCTGTAAGTACTTTCCAGCTATCTTAA
GTTTATTAGATTCCTCATTTTAGTTAGTTTGGAATTTGAAGTAAGCATGTATTTGGGGCT
TTCATCTTCTACATCCAAGAGTGATGCTTCTCTAATCTTTGAGACTGTAGAAATGATCTG
TAATTGTAAAGATATTAGCATTTAATTAGGA

Last Updated on 06.12.2018 16:11