Sequences from confirmation amplicon


Confirmation sequences for line 018D11 and hit At3g11800 are shown.
Line ID 018D11
Locus At3g11800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3727644
Primers used for PCR 03uv - 8474, show primer details
   
Sequence Name 17-K118269-0022-018-D11C-03uv
GenBank Accession LN494054 [GenBank]
Sequencing primer gene specific primer, 03uv: 5'-GAACCAAGGAGGACAAGTAGATTG-3'
Sequence (A. th genome BLAST matches underlined)
>17-K118269-0022-018-D11C-03uv
CGTTTCGGGCACGGATAACACTACACCGCGTTGAATTCGTGGTATGAGGTTGCAAATCTG
AAGCAGTACTCGCTCTATGGCCTCTACTCCAATCTAAAGGACTCACTAACCAACCCATTC
AAGAACATCTTCTGATGACTTTCTTTCTCCTTTGTGTGTTGCTACTCTTGTAATTTTGTA
TTATTTTTTCACCTGCTTTCTTTGTATTATTTCTTCCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 02-K118268-0022-018-D11C-8409
GenBank Accession LN487231 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>02-K118268-0022-018-D11C-8409
TTACATTGAATATATCCTGGAAGAAATAATACAAAGAAAGCAGGTGAAAAAATAATACAA
AATTACAAGAGTAGCAACACACAAAGGAGAAAGAAAGTCATCAGAAGATGTTCTTGAATG
GGTTGGTTAGTGAGTCCTTTAGATTGGAGTAGAGGCCATAGAGCGAGTACTGCTTCAGAT
TTGCAACCTCATACCACGAATTCAACGCGGTGTAGTGCTTATCCGTGCCCGAAAACGCCA
ATTGGATCCCCAAGCTACAATCTACTTGTCCTCCTTGGTTC

Last Updated on 06.12.2018 16:11