Sequences from confirmation amplicon


Confirmation sequences for line 018G06 and hit At3g29270 are shown.
Line ID 018G06
Locus At3g29270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11235282
Primers used for PCR DC58 - 8474, show primer details
   
Sequence Name 46-K032604-022-018-G06C-8409
GenBank Accession LN505773 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K032604-022-018-G06C-8409
GTATTAGCAGAACTGGTTAGGTCTTAGTGACAAACAAGGTATTCATGTTATCCATCCCTG
ATACTAGAATGTGGAATCTAGCATCGAGTTATCTTACGGGAAACATTGGACCGAAAAACG
ATATCAGAAGACCGGTCCATGCTCATGCGGAGTGTTCAGATGACGATGTATCTTCTGTAG
GTAGTAAAGACGAAGGTCTCGAGTGTCCTATATGCTGGGAATCATTTAATATAGTTGAAA
ACGTTCCGTATGTGTTATGGTGTGGACATACGATGTGTAAAAACTGCATTTTGGGACTTC
AATGGGCTATTGTGAAATTGCCTACTCACCCGGTTCAGCTTCCTCTCTTCATTTCATGCC
CCTGGTGCAACCTTTTGTCTTTCCGTCTGGTCTTCAGAGGAACACTCAGATTCCCTCACA
AGAACTACTTTGTGCTTTGGATGGTCGAGAGGATGCATGGAGAGA
   
Sequence Name 46-K032604-022-018-G06C-DC58
GenBank Accession LN505774 [GenBank]
Sequencing primer gene specific primer, DC58: 5'-GGTGAGTTACGTCTCTCTCCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K032604-022-018-G06C-DC58
AGTAGTTCTTGTGAGGGAATCTGAGTGTTCCTCTGAAGACCAGACGGAAAGACAAAAGGT
TGCACCAGGGGCATGAAATGAAGAGAGGAAGCTGAACCGGGTGAGTAGGCAATTTCACAA
TAGCCCATTGAAGTCCCAAAATGCAGTTTTTACACATCGTATGTCCACACCATAACACAT
ACGGAACGTTTTCAACTATATTAAATGATTCCCAGCATATAGGACACTCGAGACCTTCGT
CTTTACTACCTACAGAAGATACATCGTCATCTGAACACTCCGCATGAGCATGGACCGGTC
TTCTGATATCGTTTTTCGGTCCAATGTTTCCCGTAAGATAACTCGATGCTAGATTCCACA
TTCTAGTATCAGGGATGGATAACATGAATACCTTGTTTGTCACTAAGACCTAACCAGTTC
TGCTAATACTTAACTCTCTGACCTTCATGAACAGAGTAATTAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATTC
Primers used for PCR FP31 - 8474, show primer details
   
Sequence Name 80-K035341-022-018-G06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K035341-022-018-G06C-8409
GAAAAAAATACTCTTTTTACCTCTTTTTTTTCAATTCAAAAAA
   
Sequence Name 80-K035341-022-018-G06C-FP31
GenBank Accession LN513904 [GenBank]
Sequencing primer gene specific primer, FP31: 5'-AGAATTGCATCGGTGAGTAAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K035341-022-018-G06C-FP31
TTATCGATGAATTGTCGAGCTTTGAGCTCTAATACAAGTTAAAGCGTTCGAATATCGATT
CTGATCATAAAAATTGAATCTTGCTGTATAGTAGGTTCTTAATGATTTGTGTTACCATGG
TTGCTCAATGTAGCACTTAATCTTATTAGTTGATTCTTAGTTGGAATTGCATCGGTGTGT
CGTTGGGATTGTCTTTGAAGGATTAATATGTGTTTTCTCTTTGCTTATGAAGCGATGAGT
ATGAAAAAGAAAGAAAAAGAAAAAGAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11