Sequences from confirmation amplicon


Confirmation sequences for line 021F01 and hit At4g13800 are shown.
Line ID 021F01
Locus At4g13800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8002998
Primers used for PCR CM66 - 8474, show primer details
   
Sequence Name 08-K104111-0022-021-F01C-CM66
GenBank Accession LN489705 [GenBank]
Sequencing primer gene specific primer, CM66: 5'-AATTGAAATGTGACAATCAACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104111-0022-021-F01C-CM66
TGATTTGCAAATGCAACATATGGTCACTACTATGATGAAGATCCATGCGTGGAAGTACTT
GAATTGGTTCATCCCGGAAAAAGTCAGCTTTATGGCGATCGCCACAGCTTTAACACTCAT
AACCTAACACGCAAGAGATACTGAGACACTTACTTAAGCAAGTAGAGATATATACTAAGG
GATGGCTAAAGAAAAAGATGAAAACATACTGTAAGAGAGCCCATTAGAGAGCAAATCCCA
ACATATACTATCATATGCGTCTTCCCATAACGCGGCTCATAGTAAAAGATCAATGCAAGC
ACCACAACCAAGACCACAGCAGAATATGCAAGGAAACCTTGTTAAAACATTCAAGCAATA
GAGTATGTTTCAGTCCAATAAGAATCTTATCAATCATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 24-K104112-0022-021-F01C-8409
GenBank Accession LN496965 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K104112-0022-021-F01C-8409
TTTACAATTGAAATGATTGATAAGATTCTTATTGGACTGAAACATACTCTATTGCTTGAA
TGTTTTAACAAGGTTTCCTTGCATATTCTGCTGTGGTCTTGGTTGTGGTGCTTGCATTGA
TCTTTTACTATGAGCCGCGTTATGGGAAGACGCATATGATAGTATATGTTGGGATTTGCT
CTCTAATGGGCTCTCTTACAGTATGTTTTCATCTTTTTCTTTAGCCATCCCTTAGTATAT
ATCTCTACTTGCTTAAGTAAGTGTCTCAGTATCTCTTGCGTGTTAGGTTATGAGTGTTAA
AGCTGTGGCGATCGCCATAAAGCTGACTTTTTCCGGGATGAACCAATTCAAGTACTTCCA
CGCATGGATCTTCATCATAGTAGTGACCATATGTTGCATTTTGCAAATCAACTACTTGAA
CAAGGTAATAACAAATATTGGTTGATTGTCACAT

Last Updated on 06.12.2018 16:11