Sequences from confirmation amplicon


Confirmation sequences for line 021F02 and hit At2g26070 are shown.
Line ID 021F02
Locus At2g26070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11106420
Primers used for PCR BF43 - 8474, show primer details
   
Sequence Name 13-K029004-022-021-F02C-8409
GenBank Accession LN491902 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K029004-022-021-F02C-8409
CATTACAACAGGAGAGGAGTCCAAACTATACAGCAAGGAAATTTCGACTTCTTGGTATCA
ATCTCAGGTAGTGGCCACAGATCAGCCTCATCAGCTTCGATTATTGATGGTATAGACACG
GACACCACCCTGTCTTCAACATCATAACTTCGTTTCAGGTCCATCATAGGAACTCCTCTT
CCACGTGACATTTTTAGATTCCTAATCACACAAGACAAGAGCAGTCTGATCATTATAGTC
CAAACAAAGAGCGACCCATTTACTAAAAGAATCGAAAGTTAAAATCTTTTGATCAAAATG
AAGCAAAAGTTTCAATCTTTTTAACAAAGCATGTCCGTATATTCATTCTCACAGCACTAA
TTGTGTAGTAATCTCCTAACTTGAGCCTAATTCAGATTTTCAGCATTGTGGGTTTCTCTG
TAAATTTCACCGGAAACTAAACAAAACCCATCAAAAGCATTTCCGTAGAAAACAGTAAAA
ATCACAACAAAAAATCATAATGGAAAGCTAAGAAGCAGG
   
Sequence Name 14-K029004-022-021-F02C-BF43
GenBank Accession LN492372 [GenBank]
Sequencing primer gene specific primer, BF43: 5'-CCACTGTTATTCCCTGCTTCTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K029004-022-021-F02C-BF43
CGGAAATGCTTTTGATGGGTTTTGTTTAGTTCTCCGGTGAAATTTACAGAGAAACCCACA
ATGCTGAAAATCTGAATTAGGCTCAAGTTAGGAGATTACTACACAATTAGTGCTGTGAGA
ATGAATATACGGACATGCTTTGTTAAAAAGATTGAAACTTTTGCTTCATTTTGATCAAAA
GATTTTAACTTTCGATTCTTTTAGTAAATGGGTCGCTCTTTGTTTGGACTATAATGATCA
GACTGCTCTTGTCTTGTGTGATTAGGAATCTAAAAATGTCACGTGGAAGAGGAGTTCCTA
TGATGGACCTGAAACGAAGTTATGATGTTGAAGACAGGGTGGTGTCCGTGTCTATACCAT
CAATAATCGAAGCTGATGAGGCTGATCTGTGGCCACTACCTGAGATTGATACCAAGAAGT
CGAAATTTCCTTGCTGTATAGTTTGGACTCCTCTTCCTGTTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11