Sequences from confirmation amplicon


Confirmation sequences for line 021F05 and hit At1g44318 are shown.
Line ID 021F05
Locus At1g44318 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16832721
Primers used for PCR DC62 - 8474, show primer details
   
Sequence Name 33-K032737-022-021-F05C-8409
GenBank Accession LN500890 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K032737-022-021-F05C-8409
TTACAATTGAATATATCCTGATTAGATTGGACTAATTAGTAGAAAATAAAAAGAAGGTGA
GATTTTATGAAGTAATTTACTTGACAGAGTAAGACATAATGGACACATCTTGAAATCCCT
CAGCATCTAGAGCTGCACGAACCGCGCCTACGCGACCATCCAACATCTCACTCGTACAAA
CAACATCTGCTCCAGCTCGGGCCTGCATCAGTTCTTATTAGAATATTAAACGTTTTAGTT
ATTTAATTGGTCAAAATGGTTTCATTTTTACCTGAGAAACTGCTTGTTTCCGCAATTGGT
GAATTGTTTCATCATTTAATATCACGCCTATAAAACATAGATGGATGTTTTAGATTTACT
ACAATTTGTGAATTGGTGAATAGGTTTTTAAATGTACAAAACAGATTTCTTACCATCTTC
TCCTACGATCCCACCATGCCCAGTTGTTGAGTACTCATCAAAGTTGACATCAGTGTAGAT
AACCTTGAGAAATTAATAAGAAAGTTGTCATTATTCAAGTTCAGAACCACTTCCT
   
Sequence Name 65-K032737-022-021-F05C-DC62
GenBank Accession LN511325 [GenBank]
Sequencing primer gene specific primer, DC62: 5'-TGGTCAATAGCTAGAGGAAGTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K032737-022-021-F05C-DC62
CTTTCTATTATTTCTCAAGGTTATCTACACTGATGTCAACTTTGATGAGTACTCAACAAC
TGGGCATGGTGGGATCGTAGGAGAAGATGGTAAGAAATCTGTTTTGTACATTTAAAAACC
TATTCACCAATTCACAAATTGTAGTAAATCTAAAACATCCATCTATGTTTTATAGGCGTG
ATATTAAATGATGAAACAATTCACCAATTGCGGAAACAAGCAGTTTCTCAGGTAAAAATG
AAACCATTTTGACCAATTAAATAACTAAAACGTTTAATATTCTAATAAGAACTGATGCAG
GCCCGAGCTGGAGCAGATGTTGTTTGTACGAGTGAGATGTTGGATGGTCGCGTAGGCGCG
GTTCGTGCAGCTCTAGATGCTGAGGGATTTCAAGATGTGTCCATTATGTCTTACTCTGTC
AAGTAAATTACTTCATAAAATCTCACCTTCTTTTTATTTTCTACTAATTAGTCCAATCTA
ATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11