Sequences from confirmation amplicon


Confirmation sequences for line 021F06 and hit At3g53210 are shown.
Line ID 021F06
Locus At3g53210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19721410
Primers used for PCR RK66 - 8474, show primer details
   
Sequence Name 32-K020243-022-021-F06C-8409
GenBank Accession LN500396 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K020243-022-021-F06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATAATACGCTGATACA
AACAGAGGTCCTCCTCTTTCCACTACATATATTTGTATTGCAAACACCATTGCTGAACCA
ACTAAACCCTGAAATATATTCAAACTCACACCAAAATCAATCACACCATTCAAAAGAGTC
TTAATCTAAGAAAATAAACAAACACTTACAGTGTACAGTAACGCGTATAATTTGCCTCCT
GATATTATCTTCCACCTCTCTAGATCTCTCTCGAAATAAGCCGAAATTCCAAAGAACTGT
ATCACTGCGAAAAAGCAAGAATAGGAGACGAAGGAGAAGCGAGCTGGGTACTTTTTAAGC
AAT
   
Sequence Name 34-K020243-022-021-F06C-RK66
GenBank Accession LN501237 [GenBank]
Sequencing primer gene specific primer, RK66: 5'-CATTGCTTAAAAAGTACCCAGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K020243-022-021-F06C-RK66
CGTCTCCTATTCTTGCTTTTTCGCAGTGATACAGTTCTTTGGAATTTCGGCTTATTTCNG
AGAGAGATCTAGAGAGGTGGAAGATAATATCAGGAGGCAAATTATACGCGTTACTGTACA
CTGTAAGTGTTTGTTTATTTTCTTAGATTAAGACTCTTTTGAATGGTGTGATTGATTTTG
GTGTGAGTTTGAATATATTTCAGGGTTTAGTTGGTTCAGCAATGGTGTTTGCAATACAAA
TATATGTAGTGGAAAGAGGAGGACCTCTGTTTGTATCAGCGTATTATCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATTCACG

Last Updated on 06.12.2018 16:11