Sequences from confirmation amplicon


Confirmation sequences for line 021G04 and hit At3g17710 are shown.
Line ID 021G04
Locus At3g17710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6056226
Primers used for PCR PD01 - 8474, show primer details
   
Sequence Name 38-K021337-022-021-G04C-PD01
GenBank Accession LN502836 [GenBank]
Sequencing primer gene specific primer, PD01: 5'-TAGATGCAAGTAGCTCCAGTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K021337-022-021-G04C-PD01
CCACAACACATGATTATGGTCTTGTCAAAGATGAAGTAACTACTGCAACAATAAGTATCA
TCTAGATTAGGCAAGTTGGTTGTCGGCAATGTCATAAACTTTATCCACACCACTTCCTCC
TCTCTATCAATCTTCATCTTTGTTACCCAAATCTCAATCTTCGTTGTTTCGAAGCATTGC
TTTAACAATGAAAACCGATCTTCCTTAAAAACCGCCAGGACAAGTTCATTGCTACCAAAG
TCCTTCCGACACGGCAGTAGACAAAAGGGTTTGAAAATCTCCATGGAGAAATCAAAGGTT
TCGATGAAATACTCAAGAGTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
Primers used for PCR FL31 - 8474, show primer details
   
Sequence Name 47-K029744-022-021-G04C-8409
GenBank Accession LN506092 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K029744-022-021-G04C-8409
CATTTACAATTGAATATATCCATCAGTGTTATGAGCAAGCCAATAAAGATTCCCGTTCAC
GGACAAAGGATCCTTGTTGGGCCACAGGTTGAAAGGGTTAGGGGTATCAATAAATTTGAA
CGCCTGCGATGCACACTCGTAGATCGCAGCTTGGTAGTAACCTTCCTGGAGAGAGTCGCT
GAGTCTACGCGTACAATTAAAATACCCCAAGATCTTGTAACACTTATCGGGTTTACAACT
ATCGTATCCTACACCGCAAAAACGGAAACCTTTATCCTCATACTCGATCCACCCAACCTG
TCTCAACCACGGATTCCAAACCGCAACACCTTGTTTCCAAAAATCACGAAACAATAACCC
ATCGCAAGCCATGATAGTTGTAAAATTCTTCTTCATAGGCTGACAATGAAAATCGTAGGG
TACCTCACGCACCTCTATCGTTCCACCCAGATCGATCTCTATCGAGTACATCTTCTTCGA
TTCGGTAAGGAAGATGAATTGGGGACGGGCACGGACCAAGTGCTTTTTGACAAACCTCTT
GTCGCTGAAAAGACCGTTCCAG
   
Sequence Name 48-K029744-022-021-G04C-FL31
GenBank Accession LN506405 [GenBank]
Sequencing primer gene specific primer, FL31: 5'-AGGACCGTATGTAAACACTGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K029744-022-021-G04C-FL31
AAGCACTTGGTCCGTGCCCGTCCCCAATTCATCTTCCTTACCGAATCGAAGAAGATGTAC
TCGATAGAGATCGATCTGGGTGGAACGATAGAGGTGCGTGAGGTACCCTACGATTTTCAT
TGTCAGCCTATGAAGAAGAATTTTACAACTATCATGGCTTGCGATGGGTTATTGTTTCGT
GATTTTTGGAAACAAGGTGTTGCGGTTTGGAATCCGTGGTTGAGACAGGTTGGGTGGATC
GAGTATGAGGATAAAGGTTTCCGTTTTTGCGGTGTAGGATACGATAGTTGTAAACCCGAT
AAGTGTTACAAGATCTTGGGGTATTTTAATTGTACGCGTAGACTCAGCGACTCTCTCCAG
GAAGGTTACTACCAAGCTGCGATCTACGAGTGTGCATCGCAGGCGTTCAAATTTATTGAT
ACCCCTAACCCTTTCAACCTGTGGCCCAACAAGGATCCTTTGTCCGTGAACGGGAATCTT
TATTGGCTTGCTCATAACACTGATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11