Sequences from confirmation amplicon


Confirmation sequences for line 022A04 and hit At1g73655 are shown.
Line ID 022A04
Locus At1g73655 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27690483
Primers used for PCR RK36 - 8474, show primer details
   
Sequence Name 45-K015904-022-022-A04C-8409
GenBank Accession LN505357 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K015904-022-022-A04C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGAAACAATAAACTGTATC
AACCTCGATGATATAGTCAAGTGTTGCAGATGGAGGAATCTCCAAACCCTGTCCAAATTC
GACATTTCTGTCTCCAAATCCTAAGGACGGTGGAATTATCACTCTACGTTTACCTCCAGC
CTTCATTGACCTGAGAACATGCTCTATACCTTGGCATAGTCCCTTGCTATACGGTCTTGA
GTCCATTACCATCGCTAGTGACTTGCCTTTGCCTCCAAATGTGTCCACGAACACTTGTTC
AGTGCCATGTACTTGTCCCTTTACATCAAACACTACCAAGTATCCTGAGCTTGG
   
Sequence Name 46-K015904-022-022-A04C-RK36
GenBank Accession LN505704 [GenBank]
Sequencing primer gene specific primer, RK36: 5'-TCCAAGCTCAGGATACTTGGTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K015904-022-022-A04C-rk36
GTTTGATGTAAAGGGACAAGTACATGGCACTGAACAAGTGTTCGTGGACACATTTGGAGG
CAAAGGCAAGTCACTAGCGATGGTAATGGACTCAAGACCGTATAGCAAGGGACTATGCCA
AGGTATAGAGCATGTTCTCAGGTCAATGAAGGCTGGAGGTAAACGTAGAGTGATAATTCC
ACCGTCCTTAGGATTTGGAGACAGAAATGTCGAATTTGGACAGGGTTTGGAGATTCCTCC
ATCTGCAACACTTGACTATATCATCGAGGTTGATACAGTTTATTGTTTCATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGGTCTACA
TTCA

Last Updated on 06.12.2018 16:11