Sequences from confirmation amplicon


Confirmation sequences for line 022D05 and hit At4g23690 are shown.
Line ID 022D05
Locus At4g23690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12339458
Primers used for PCR DC66 - 8474, show primer details
   
Sequence Name 51-K032605-022-022-D05C-8409
GenBank Accession LN507405 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K032605-022-022-D05C-8409
TTGAATATATCAAAGATCACAAACTTACCGAACTTGAAGTTTCCTAGTCCTGGAGGGCTC
ACGATGGCGGCGGAGGTTGCGTTTGCTACATTGTCGCCATCGTAGAGGATGTCATGGAAA
TAGAACGAGAAGTGTTTGCAGGGTTTTTTCTGGTCGATTGTTTTTCGGAAAGATAAGACG
GTATCGGAGAAGAGTAAAACTAATAAGAAGAATGAGAAGAGGGCTTTGAAGAGTTGTTTC
TCTACTAGAAATGCCATGGTTAGCTAGAGATTAGTGAGTTGTGAGATGTGTTTAATGAAG
GAGCCTTTAAACCCAATCTCACAACTCACTAATCTCTAGCTAACCATGGCATTTCTAGTA
GAGAAACAACTCTTCAAAGCCCT
   
Sequence Name 51-K032605-022-022-D05C-DC66
GenBank Accession LN507406 [GenBank]
Sequencing primer gene specific primer, DC66: 5'-GGGTTTAAGCCTCCTTCATTAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K032605-022-022-D05C-DC66
GGCATTTCAGTAGAGAAACAACTCTTCAAGCCCTCTTCTCATTCTTCTTATTAGTTTTAC
TCTTCTCCGATACCGTCTTATCTTTCCGAAAAACAATCGACCAGAAAAAACCCTGCAAAC
ACTTCTCGTTCTATTTCCATGACATCCTCTACGATGGCGACAATGTAGCAAACGCAACCT
CCGCCGCCATCGTGAGCCCTCCAGGACTAGGAAACTTCAAGTTCGGTAAGTTTGTGATCT
TTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTC
Primers used for PCR DC65 - 8474, show primer details
   
Sequence Name 82-K032614-022-022-D05C-DC65
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, DC65: 5'-CGGAAATACTTATCCCCTTGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K032614-022-022-D05C-DC65
CCTGGATCGTCCATGGTCCTGATGGGCACCATGGAGAAAAAGACCGAGTAGTTACCAATT
TTTTTTCTTTTCATCCATGTACATGTCCGCAACGTTATTATCTTGTGAACGTACCTTTTG
ATAAAACGACTAATTACGATCCGTCGTATTTGTAGGCGAAAGCTGTAAACGGATTATTCT
AATGCAGAAATCTTTGTTTCGACGTGTCTACATTCGCGCCCATAATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 82-K032614-022-022-D05C-8409
GenBank Accession LN514133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K032614-022-022-D05C-8409
TTGAATATATCCTGAACAATGGACAAGAACTATCTCTCAAAACCCGTGGCTCGCGCACAA
GGCTTCTATTTCTATGACATGAAGATGGACTTCAATTCGTGGTTTTCCTACACGTTGGTG
TTTAACTCAACGGAACACAAAGGGACATCGAACATAATGGGTGCTGATTTGATGATGGAG
CCAACAAGAGATCTATCGGTCGTTGGTGGGACAGGTGACTTCTTCATGGCTCGTGGGATC
GCTACCTTCGTGACTGATTTATTTCAAGGGGCTAAGTATTTCCGAGTTAAGATGGATATT
AAGCTCTATGAATGTTACTAAGTAGCTAAGAATGTGTGTTTTGTTTGAAAATTTTATGTT
GACACAGAGAAATGTGTTTACAATAATTAAGTTTTTTTTTTGCCTTTTCTTTTGCTGTTG
TCTTTTTGTTTATCTATAATTATTATGTGATTTATTTCAAGG

Last Updated on 06.12.2018 16:11