Sequences from confirmation amplicon


Confirmation sequences for line 022G05 and hit At2g44450 are shown.
Line ID 022G05
Locus At2g44450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18343689
Primers used for PCR VL02 - 8474, show primer details
   
Sequence Name 02-K017366-022-022-G05C-VL02
GenBank Accession LN486821 [GenBank]
Sequencing primer gene specific primer, VL02: 5'-CATTGGTCCAAAGGTACTTGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K017366-022-022-G05C-vl2
CAAACAACTTGCAATGGTTATCAATATTTGCGAACTTATTCAAACTCAAGCGCTAAGCTA
TGTGTGGGGTTTTATTTTATAGGCTGCATCAGATTGGCTTTTAATATATCCAAAGGGAAT
TCGTGATCTTGTGCTCTATGCAAAATACAAGTTTAAGGACCCTGTCATGTACATAACTGA
AAACGGTAACTCACACAAACAATTGCTTCAGTTCTTGATACATATATTCCTTTAGTATCC
TAATGACGTATATAACAATCTTTGTAGGGAGAGATGAATTTAGTACCAATAAAATCTTCC
TTAAAGACGGTGACAGGATCGATTACTACGCTCGACATCTCGAAATGGGTTCAAAAGCTT
TCTCGTAAGCATCTTTTTATATCTAGTGTTAAGACTCCCAATTCGAAATAAACTATCATT
CATATTTATGTAATTATGTTGCAAATGTAGTGTTGGAGCCAATGTGAAGGGATTTTTCGC
ATGGTCGTTGCTAAACAATTTCGAATGGGCAA
   
Sequence Name 06-K017366-022-022-G05C-8409
GenBank Accession LN488587 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K017366-022-022-G05C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGCTAATACGAGTTGACATTGTCCTCTATT
TACATCCGTCTTTGAAATCCACATAAACCAGTCCAAACCGGACCGTGTATCCCATTGCCC
ATTCGAAATTGTCTAGCAACGACCATGCGAAAAATCCCTTCACATTGGCTCCAACACTAC
ATTTGCAACATAATTACATAAATATGAATGATAGTTTATTTCGAATTGGGAGTCTTAACA
CTAGATATAAAAAGATGCTTACGAGATAGCGTCTTGAACCATTTCGAGATGTCGAGCGTA
GTAATCGATCCTGTCACCGTCTTTAAGGAAGATTTTATTGGTACTAAATTCATCTCTCCC
TACAAAGATTGTTATATACGTCATTAGGATACTAAAGGAATATATGTATCAAGAACTGAA
GCAATTGTTTGTGTGAGTTACCGTTTTCAGTTATGTACATGACAGGGTCCTTAAACTTGT
ATTTTGCATAGAGCACAAGATCACGAATTCCCTTTGGATATATTAAAAGCCAATCTGATG
CAGCCTATAAAATAAAACCCCACACATAGCTTAGCGCTTGAGTTTGAATAAGTTCGCAAA
TATTGATAACCATTGCAAGTTGTTTGTGTCTAAAA
Primers used for PCR FL35 - 3144, show primer details
   
Sequence Name 90-K028756-022-022-G05C-3144
GenBank Accession LN514996 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K028756-022-022-G05C-3144
TCTATATAAGGGAAGTTTCATTTTCATTTGCAGTGGAACGGGTACCCGGGGAATCAGAAT
GTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAATTATCTGCCTAACCGGCTCAC
TTCTGCTTCTGTATTTTTGATTTCCAAGAAATCAGCTGAATGGTTCAGAAAGTTATTGAA
TGAAAAGAAAAACGATTGATGATGATGAGATATGTATATTTCATTATATTAGTTTCTCTG
TTACTCTTGAGAGATTTATATATTTTTTCCCCCCTTTGGAAATAATGAGAAATGGTTTTA
GCTATTTTCACAACGAAACCCGATTGATGTATAGCTGATGGAAATCATCTTTCGATGTAA
TTACGTACGTTTTATAAGGAAACTTAATATTAAACTTGTTGAAAAAGTTAAAAAAAAAAT
AGAATGTGAAAACGATATAGGCAAAGAGTATGTAACATCTACAACTTTCAGCATATATCA
ATTTGAAAAAAAATGTTCAATCTTTAAATTTTTGAAGAATCTCATGGTTTTCAAAGATCA
TCATTTATGGTATTATGAGTTGGATTATTGATATTTTTTTAAAGATTGTGATGGT
   
Sequence Name 91-K028756-022-022-G05C-FL35
GenBank Accession LN515080 [GenBank]
Sequencing primer gene specific primer, FL35: 5'-AATCAATAATGGGACCATCACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K028756-022-022-G05C-FL35
CACATCTCTATTTTTTTTTTAACTTTTTCAACAAGTTTAATATTAAGTTTCCTTATAAAA
CGTACGTAATTACATCGAAAGATGATTTCCATCAGCTATACATCAATCGGGTTTCGTTGT
GAAAATAGCTAAAACCATTTCTCATTATTTCCAAAGGGGGGAAAAAATATATAAATCTCT
CAAGAGTAACAGAGAAACTAATATAATGAAATATACATATCTCATCATCATCAATCGTTT
TTCTTTTCATTCAATAACTTTCTGAACCATTCAGCTGATTTCTTGGAAATCAAAAATACA
GAAGCAGAAGTGAGCCGGTTAGGCAGATAATTTAAACACTGATAGTTTAAACCGAAGGCG
GGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTAT
ATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAG
ATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATG
CTCCTCGTGGG

Last Updated on 06.12.2018 16:11