Sequences from confirmation amplicon


Confirmation sequences for line 023B05 and hit At4g04760 are shown.
Line ID 023B05
Locus At4g04760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2427483
Primers used for PCR RK29 - 8474, show primer details
   
Sequence Name 26-K016432-022-023-B05C-8409
GenBank Accession LN497695 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K016432-022-023-B05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAGGTAAAAATCTTTAATACAATTGAACT
AAAGTTGACAAAAAGTTGATTTGGTTACAATTTCTGAATTTTGAAAAGAGAGTTGTTAAC
TAAAAGTCTTTGTTGCAGGAACATTTCTGATGTTTGCCACAGTGGCTGGCCTTGGATTTG
TGTTCATAGCCAAGCTTGTTCCAGAGACCAAAGGCAAATCTCTAGAAGAGATTCAATCTC
TTTTCACTGATTCTCCTCCTCAAGATTCTACAATTTTCTAAACTTCTCCCACAACTGTAT
AAGATAGAAGTATGTAAACAAAAAAAGTTTATTGCAATATAGTTTTTACGTGATTCACAA
ATATTAACAAACACCTTTCACAAGATTCACATGAAACTACCAAAACATTACAAAATAATA
CAAAGTTTTACTCAGAAGAAAAGCTTTATTGAGAAGAAGCACAAAGACCACCCCTTTTTT
TTTGCTGTTCTGTAATTATGAGTTTTGTTTTAACCCACTGATCCTTCAAGCTTGATGCTC
ATAAGCTGGTTAACTTCAGCTCCAAACTCATCAGGTAACTTGTTGAAAACATCTCTGCA
   
Sequence Name 30-K016432-022-023-B05C-RK29
GenBank Accession LN499510 [GenBank]
Sequencing primer gene specific primer, RK29: 5'-GATTTCCACTTTTGTTTTGTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K016432-022-023-B05C-rk29
CTGGAACAATAACAGGTAAAGAATCCATCGGCGAAAGTAGAGCACGAAGCGAGTACCTCT
AAGATTGGAGAAGATCAGCTATTCTATTTCCAGCAGAGAGGAATCGATCACGAGAGAGCA
TTAGCGGCAATGATCTCTGGTTTCTGCAGAGATGTTTTCAACAAGTTACCTGATGAGTTT
GGAGCTGAAGTTAACCAGCTTATGAGCATCAAGCTTGAAGGATCAGTGGGTTAAAACAAA
ACTCATAATTACAGAACAGCAAAAAAAAAGGGGTGGTCTTTGTGCTTCTTCTCAATAAAG
CTTTTCTTCTGAGTAAAACTTTGTATTATTTTGTAATGTTTTGGTAGTTTCATGTGAATC
TTGTGAAAGGTGTTTGTTAATATTTGTGAATCACGTAAAAACTATATTGCAATAAACTTT
TTTTGTTTACATACTTCTATCTTATACAGTTGTGGGAGAAGTTTAGAAAATTGTAGAATC
TTGAGGAGGAGAATCAGTGAAAAGAGATTGAATCTCTTCTAGAGATTTGCCTTTGGTCTC
TGGAACAAGCTTGGCTATGAACACAAATCCAAGGCCAGCCACTGTGGCAAACATCAGAAG
TGTTCCTGCAACAAAGACTTTTAGTTAACAACTCTCTTTTCAAAATTCAGAAATTGTAAC
CAAATCAACTTTTTGTCAACTTTAGTTCAATTGTATTAAAGATTTTTACCTGTA
Primers used for PCR FL38 - 8474, show primer details
   
Sequence Name 01-K035332-022-023-B05C-8409
GenBank Accession LN486473 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K035332-022-023-B05C-8409
GAGCCATTTAAATTGAATATATTCAACAAGCCATGCGCTTATAGAGCTGACCAAGTTACA
CATTGTTCCCGCTGCTCCCTTAACATCTACTGGATAAATCTTGAAAAGAAAATAAAAACA
TTAAGAGGGATGTATTCAATCTAGTATTTTAAGTTATTTGTGTAAAATTTACAAATCCGA
TGTTATTCAATCACGGATTTTAAAAACTCTAATGAAATTCAGTGTTATTGAACTAATGAT
TTAAAAATCTAGATGTATGCGATACATAATTGTTATAATGAGTGTAACATAATTTAAATG
TCTGCGATATATTTTTAAACTTTTTTTAAATATTTATTTACCTCTGATGCTATGATCCAT
GGTATTG
   
Sequence Name 01-K035332-022-023-B05C-FL38
GenBank Accession LN486474 [GenBank]
Sequencing primer gene specific primer, FL38: 5'-CAATACCATGGATCATAGCATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K035332-022-023-B05C-FL38
TAAAAGTTTAAAATATATCGCAGACATTTAAATTATGTTACACTCATTATAACAATTATG
TATCGCATACATCTAGATTTTTAAATCATTAGTTCAATAACACTGAATTTCATTAGAGTT
TTTAAAATCCGTGATTGAATAACATCGGATTTGTAAATTTTACACAAATAACTTAAAATA
CTAGATTGAATACATCCCTCTTAATGTTTTTATTTTCTTTTCAAGATTTATCCAGTAGAT
GTTAAGGGAGCAGCGGGAACAATGTGTAACTTGGTCAGCTCTATAAGCGCATGGCTTGTT
GAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11