Sequences from confirmation amplicon


Confirmation sequences for line 024A07 and hit At3g26300 are shown.
Line ID 024A07
Locus At3g26300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9639275
Primers used for PCR MS52 - 8474, show primer details
   
Sequence Name 53-K017403-022-024-A07C-MS52
GenBank Accession LN507987 [GenBank]
Sequencing primer gene specific primer, MS52: 5'-AAACTTTCTCACTCGCAAAATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K017403-022-024-A07C-ms52
GGTAGCTACCCATTCATGTTGATATGTATGAATATCTCTTTTGACGAGTGAAATCTGTGT
AAGGTTTCAGTCGTTTTATTCTGATTTGTTTTTCAAATCATGATCATACAAGAGTACAAG
ATCATCCCATCTTAGAACCAAATTCTTGAGAGCACAATATTACAAAACGTAATGCGTTAA
AACATTTTATTTAAAAAGAAGAAACTCTTTTTTTTTGGCCTGTCTGATTAAACATCAAAG
GAAGATGCGATGTTTTTATATATTCCATGTGATGACATTATTTTAAGGATCTAGAAACTT
CGTTGGCACAAGTATAAGCTCGTTTTTCTTGTTGACAGTGAGTCCGGGAGCTTCTTCCAA
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTCA
   
Sequence Name 54-K017403-022-024-A07C-8409
GenBank Accession LN508325 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K017403-022-024-A07C-8409
ATTTTCCGGACATGAAGCCATTTACAATTGATTGGAAGAAGCTCCCGGACTCACTGTCAA
CAAGAAAAACGAGCTTATACTTGTGCCAACGAAGTTTCTAGATCCTTAAAATAATGTCAT
CACATGGAATATATAAAAACATCGCATCTTCCTTTGATGTTTAATCAGACAGGACAAAAA
AAAAGAGTTTCTTCTTTTTAAATAAAATGTTTTAACGCATTACGTTTTGTAATATTGTGC
TCTCAAGAATTTGGTTCTAAGATGGGATGATCTTGTACTCTTGTATGATCATGATTTGAA
AAACAAATCAGAATAAAACGACTGAAACCTTACACAGATTTCACTCGTCAAAAGAGATAT
TCATACATATCAACATGAATGGGTTGCTACCGATAATCCAAAATTTTCCCATATGTTGGA
ATGATTTTGCGAGTG
Primers used for PCR FL39 - 8474, show primer details
   
Sequence Name 20-K029743-022-024-A07C-FL39
GenBank Accession LN495086 [GenBank]
Sequencing primer gene specific primer, FL39: 5'-ATGGTTATCAAAGAAACATGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K029743-022-024-A07C-FL39
ATCCCAAGGGAAGCAATGTCTGAATTTGAGATCAACGGCTACACGATTCCAGTTAAGACA
CGGCTTCACGTGAATGTATGGGCTATTGGTCGTGATCCCGATACCTGGAAAGACCCTGAA
GTGTTTCTCCCGGAGAGGTTTACGGATAATAATATTGATGCAAAAGGACAGCATTTTGAG
CTGTTACCGTTTGGAGGCGGCCGGAGAATGTGTCCTGCAGTTTATATGGGGACAACAATG
GTCGAGTTTGGTTTGGCTAATCTTTTGTATCATTTTGATTGGAAGTTACCAGAAGGCATG
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11