Sequences from confirmation amplicon


Confirmation sequences for line 024B02 and hit T10C21 are shown.
Line ID 024B02
Locus T10C21 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14977880
Primers used for PCR NE25 - 8474, show primer details
   
Sequence Name 52-K102433-0022-024-B02C-8409
GenBank Accession LN507773 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K102433-0022-024-B02C-8409
ATTTACATTGAATATTATGCTGTGTTGGATCAAACTCTCCAGAACCAAGATATAGAGACC
ATTTTACCTGCTTGGATAACAAAGATATAGAGACCATTTTACCTGCTTGAATAACGGGTT
TTGTAGTGATACACAGAAACTAGATTGAAGAACGAGAGACAAGGGAAAATCCATTTTGCT
TCTCTATAGCAATCTGAAGAAAACCACAGCAAAAAAAGTACCATATATAGAGAGGCTCGA
ACACAATTTCCAGCTCATTTCAAACAAAAGTATCTCTCTAACCGAAGATAACAAAGTTAC
TTCTAAACAAAAAGGATCTAAGGATCTAAGTCTCTCTCAAGTGATCCAAGTCTCTTCAGT
TTCTTAGAGTAGGTTTCTCAGGAGTGCCTGCCAACGAAGATGCACATTATTTGAAAATCT
TGGACCTCCTCTATC
   
Sequence Name 52-K102434-0022-024-B02C-NE25
GenBank Accession LN507774 [GenBank]
Sequencing primer gene specific primer, NE25: 5'-AGAAAGAGGAGGTCCAAGATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K102434-0022-024-B02C-NE25
TCCTGAGAACCTACTCTAGAAACTGAAGAGACTTGGATCACTTGAGAGAGACTTAGATCC
TTAGATCCTTTTTGTTTAGAAGTAACTTTGTTATCTTCGGTTAGAGAGATACTTTTGTTT
GAAATGAGCTGGAAATTGTGTTCGAGCCTCTCTATATATGGTACTTTTTTTGCTGTGGTT
TTCTTCAGATTGCTATAGAGAAGCAAAATGGATTTTCCCTTGTCTCTCGTTCTTCAATCT
AGTTTCTGTGTATCACTACAAAACCCGTTATTCAAGCAGGTAAAATGGTCTCTATATCTT
TGTTATCCAAGCAGGTAAAATGGTCTCTATATCTTGGTTCTGGAGAGTTTGATCCAACAC
AGCATATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11