Sequences from confirmation amplicon


Confirmation sequences for line 024F06 and hit At2g40150 are shown.
Line ID 024F06
Locus At2g40150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16776038
Primers used for PCR BA42 - 8474, show primer details
   
Sequence Name 39-K104830-0022-024-F06C-BA42
GenBank Accession LN503344 [GenBank]
Sequencing primer gene specific primer, BA42: 5'-ACATGCTCATGAAGAAAACAGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K104830-0022-024-F06C-BA42
ATCTCCAATGTCCTCAACACCCTCTCGTACGCTATTGGCCGTTTGATCTCATCATACTCT
GTGTCCCCATCATCGAACGATCCACGTCTGTTTCCATCATCACAAATCTTCAATCCTAGG
ATATTCAATACATAAGTATGTGTGAGTGTGTATACTATAACATTACTACTTACAGGACTT
TGATGGAGACAGTATTCATCCACCAGATGTAACTATTGAAGACAAGAAAGTCAACGCCTA
TCCAGTTGACTCCATGCTTCTCGATGGACTCTGGCATTATTATACGGTCGATTATACTGT
GCTTTTCCGGATCGTCTGAATTTGATTCCACCAAAAATGGCGCCCAATAAAACTCCACCG
TCGCGTTATAGTCCTATTTTAAATTTCAGTTTTGCATATCCCAAGAAAGTAAGAAACTAT
AAAAATAATGTCATTAGTGTCAGTGTCACACAAGTGGATTAATGTTTATTACCTGGATTT
TGAAAACAGTGAGTGAACCGGTCTGGTTTAAGCTTTTTCTACCGGGAGGAATCACTGATT
GAACCAAACAAACCATTGATTCCCATTGGTTCCGGTTTAACGAGTCACCGACAGCAATAT
GTCCGGGAAATCTTCATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 54-K104831-0022-024-F06C-8409
GenBank Accession LN508471 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K104831-0022-024-F06C-8409
ACTCGTTAACCGGAACCAATGGGAATCAATGGTTTGTTTGGTTCAATCAGTGATTCCTCC
CGGTAGAAAAAGCTTAAACCAGACCGGTTCACTCACTGTTTTCAAAATCCAGGTAATAAA
CATTAATCCACTTGTGTGACACTGACACTAATGACATTATTTTTATAGTTTCTTACTTTC
TTGGGATATGCAAAACTGAAATTTAAAATAGGACTATAACGCGACGGTGGAGTTTTATTG
GGCGCCATTTTTGGTGGAATCAAATTCAGACGATCCGGAAAAGCACAGTATAATCGACCG
TATAATAATGCCAGAGTCCATCGAGAAGCATGGAGTCAACTGGATAGGCGTTGACTTTCT
TGTCTTCAATAGTTACATCTGGTGGATGAATACTGTCTCCATCAAAGTCCTGTAAGTAGT
AATGTTATAGTATACACACTCACACATACTTATGTATTGAATATCCTAGGATTGAAGATT
TGTGATGATGGAAACAGACGTGGATCGTTCGATGATGGGGACACAGAGTATGATGAGATC
AAACGGCCAATAGCGTACGAGAGGGTGTTGAGGACATTGGGAGATTGGGTGGACCATAAC
ATTGATCCTCTAAGTACAACTGTTTT

Last Updated on 06.12.2018 16:11