Sequences from confirmation amplicon


Confirmation sequences for line 025A01 and hit At3g13030 are shown.
Line ID 025A01
Locus At3g13030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4170666
Primers used for PCR 51lx - 8474, show primer details
   
Sequence Name 54-K114129-0022-025-A01C-51lx
GenBank Accession LN508549 [GenBank]
Sequencing primer gene specific primer, 51lx: 5'-TGTGGAGAAGATTAAAGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K114129-0022-025-A01C-51lx
GCTTGGGTTGACAGAAAGGCCGTGATCTTGTTACTTTTGTTGCAGATTGTCCAGCGGGTT
TAGTGTATCTGATATCATTTGATGTTTCAGATTTTAAAGACGATGTCACTGCCTTACTAT
CGCTAGTAAATGGGCTTGTTGAGGAAGTTGGAGTGCGTAATGTGACTCAAATTATTGCAT
GTTCAACCTCTGGTTGGGTTGGTGAATTGGGTGAGCTATTTGCGGGTCATGACAGGGAAG
TTTTCTGGTCTGTGAGTGTATCCCATTGTTTCGAGCTTATGCTGGTGAAGATTAGCAAAA
TACGTTCATTTGGAGACATATTTGACAAAGTCAATAACATCTGGTTGTTCATCAACAACT
GAACATTCCCCGGGATCAATTATGACAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 12-K114128-0022-025-A01C-oriL
GenBank Accession LN491667 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114128-0022-025-A01C-oriL
CATTTACAATTGAATATATCCTGTCATAATTGATCCCGGGGAATGTTCAGTTGTTGATGA
ACAACCAGATGTTATTGACTTTGTCAAATATGTCTCCAAATGAACGTATTTTGCTAATCT
TCACCAGCATAAGCTCGAAACAATGGGATACACTCACAGACCAGAAAACTTCCCTGTCAT
GACCCGCAAATAGCTCACCCAATTCACCAACCCAACCAGAGGTTGAACATGCAATAATTT
GAGTCACATTACGCACTCCAACTTCCTCAACAAGCCCATTTACTAGCGATAGTAAGGCAG
TGACATCGTCTTTAAAATCTGAAACATCAAATGATATCAGATACACTAAACCCGCTGGAC
AATCTGCAACAAAAGTAACAAGATCACGGCCTTTCTGGTCAACCCAAGCATCCAACAAGA
TGCTGCATCCTGTGATCGCCCACGATTCTTTAATCTTCTCCAC
Primers used for PCR 74wk - 8474, show primer details
   
Sequence Name 45-K118366-0022-025-A01C-74wk
GenBank Accession LN505682 [GenBank]
Sequencing primer gene specific primer, 74wk: 5'-TTTGAATTGAACGTGACAATCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118366-0022-025-A01C-74wk
ACACTTCTATATCAAGATGGAAATTTGTTGAGTAGAAAGCAGTCGGGTTCAGAAAGTAAC
CAGCAGCATGAAGAGGATTGTGAAGATGCTTGTTCCAAACATCATCTATCACATCCCATA
ACGGCTTGTAAAATTGTGGCTTGTGATTGAATTCTCTCGCGATACTCTCCTTTATGCTGT
CCATAGTGTCATAAACATATCCAAGATGCTGATTGTTGGCAGTTGAAAACAAGAGCAGAC
CGTGAATCAGAGGAGATGTACATTTCAGAACACTTTCAACTGTTTCCCAAAAAGAGGAGT
CACTCACCAAATTTGATATTGCTATGCACTGTTCGTTATTCCAATTAGATGAGGCAAACA
TGGCTGTCAAGTTTTTCTTCGCCTTGAAAATACTCTCTAGAATTAGATATGGCGTCACAA
ACTCAAACTCAGAGGAGGAGACTGTTATGTCTATTCCATGACACTGATCTCTGAAAATGT
TCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATATCCGCAG
   
Sequence Name 66-K118365-0022-025-A01C-8409
GenBank Accession LN511703 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K118365-0022-025-A01C-8409
TTACATTGAATATGAACATTTTCAGAGATCAGTGTCATGGAATAGACATAACAGTCTCCT
CCTCTGAGTTTGAGTTTGTGACGCCATATCTAATTCTAGAGAGTATTTTCAAGGCGAAGA
AAAACTTGACAGCCATGTTTGCCTCATCTAATTGGAATAACGAACAGTGCATAGCAATAT
CAAATTTGGTGAGTGACTCCTCTTTTTGGGAAACAGTTGAAAGTGTTCTGAAATGTACAT
CTCCTCTGATTCACGGTCTGCTCTTGTTTTCAACTGCCAACAATCAGCATCTTGGATATG
TTTATGACACTATGGACAGCATAAAGGAGAGTATCGCGAGAGAATTCAATCACAAGCCAC
AATTTTACAAGCCGTTATGGGATGTGATAGATGATGTTTGGAACAAGCATCTTCACAATC
CTCTTCATGCTGCTGGTTACTTTCTGAACCCGACTGCTTTCTACTCAACAAATTTCCATC
TTGATATAGAAGTTGTCACTGGTCTTATCTCCTCTCTGATTCATATGGTAGAAGATTGTC
ACGTCAAATTCAA

Last Updated on 06.12.2018 16:11