Sequences from confirmation amplicon


Confirmation sequences for line 025E12 and hit At4g28370 are shown.
Line ID 025E12
Locus At4g28370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14037821
Primers used for PCR 02vh - 8474, show primer details
   
Sequence Name 81-K117997-0022-025-E12C-02vh
GenBank Accession LN514114 [GenBank]
Sequencing primer gene specific primer, 02vh: 5'-ATGAAACATCAGCATTAGCATCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K117997-0022-025-E12C-02vh
ACAGAAGTCACATAAATGGTGAGAATATCTAGCACTCAAGATTCAGTGCAGGTTATTGTC
CTTAGAATCAAAATAAAACCTACATAAACTAGTGAATAACTTGGAAATTTTGAGAAGATA
AACAAAACCATTTTTCCATACCATAGTGACAGGATATACTCGTATAGTTTCCAAAAATGC
AATTTTCTCATTGGGATATTACATTTTTCTCTGTGGTTTGGAAGACAATTCAAACCATTT
TCTTGGTATTGGTTTCAAGGAAAATAGCATTGAAAAGAGGGTAAGAGAATCAAGCAGTTA
TATATGACCCATATCATAAACATGCAGTAAGAAAAGAGTTTGAAGCTTTAATCGATAAAA
AAAACACCAAAGAAGTAAGTACTCACAGAAACGACTGTACAGAAAGGACAGTTCTCTCCT
CACAGAAACTGGCTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATATCCGCACGGTT
   
Sequence Name 41-K117996-0022-025-E12C-8409
GenBank Accession LN504247 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117996-0022-025-E12C-8409
ATTTACATTGAATATTAGCCAGTTTCTGTGAGGAGAGAACTGTCCTTTCTGTACAGTCGT
TTCTGTGAGTACTTACTTCTTTGGTGTTTTTTTTATCGATTAAAGCTTCAAACTCTTTTC
TTACTGCATGTTTATGATATGGGTCATATATAACTGCTTGATTCTCTTACCCTCTTTTCA
ATGCTATTTTCCTTGAAACCAATACCAAGAAAATGGTTTGAATTGTCTTCCAAACCACAG
AGAAAAATGTAATATCCCAATGAGAAAATTGCATTTTTGGAAACTATACGAGTATATCCT
GTCACTATGGTATGGAAAAATGGTTTTGTTTATCTTCTCAAAATTTCCAAGTTATTCACT
AGTTTATGTAGGTTTTATTTTGATTCTAAGGACAATAACCTGCACTGAATCTTGAGTGCT
AGATATTCTCACCATTTATGTGACTTCTGTTTTTCATTTAGCTGAAACTTTGACGCANGT
TCAGATGCTAATGCTGATATTTCAT

Last Updated on 06.12.2018 16:11