Sequences from confirmation amplicon


Confirmation sequences for line 025H01 and hit At2g31010 are shown.
Line ID 025H01
Locus At2g31010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13197540
Primers used for PCR 01ie - 8474, show primer details
   
Sequence Name 58-K112758-0022-025-H01C-01ie
GenBank Accession LN509751 [GenBank]
Sequencing primer gene specific primer, 01ie: 5'-TTGAGCACTGGTAGGAATTCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K112758-0022-025-H01C-01ie
GGTACTCATTTTACATTAGTGCATGCGAAAATTTTCAGTATGCCGCTCCTATCATATACA
TTCCAGTATTAGTTAAGTTGCTTATACAAGTCTGCATGTATATATTTGATTGGCTGTGCA
AGCAATTTTTTAATATTCTCTAGCTTTCAAACAGTTCATCCACATCAGAAATTCGTAAAA
CTAGACGGCGAAGTTTTAGGATCACACCAGAAATTGGCGATGACATTGCAAGGTTGGTAT
TCTCATCCATGTGCATTGTTTCTACTTTCATCGTTTAGCAGTTTTTTTCTATCATTTTCA
CCTTGCATTTAAAGAGCTCTATATTGGAGTTTATTCTGTAAATCTAGTTGGTAAGGTATT
TTTAAAATCTACAATCATCTGAGCAGCTGCTAGATCACATGAAAGTATGGCATGAGTCAT
CTCGAATGCAAAACTGCTATCTCCAAACTTCTAGATGCTTAGGTTAGGTGTCTGTAAGAT
GGTTATATGATAAACATGGAACTATATATTTACTTTGATAGTCATAGATTGAGAAAAAGA
AATCTACATGGATCAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 18-K112757-0022-025-H01C-8409
GenBank Accession LN494377 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K112757-0022-025-H01C-8409
TTCATTGAATATATCCTGTTGATCCTGTAGATTTCTTTTTCTCAATCTATGACTATCAAA
GTAAATATATAGTTCCATGTTTATCATATAACCATCTTACAGACACCTAACCTAAGCATC
TAGAAGTTTGGAGATAGCAGTTTTGCATTCGAGATGACTCATGCCATACTTTCATGTGAT
CTAGCAGCTGCTCAGATGATTGTAGATTTTAAAAATACCTTACCAACTAGATTTACAGAA
TAAACTCCAATATAGAGCTCTTTAAATGCAAGGTGAAAATGATAGAAAAAAACTGCTAAA
CGATGAAAGTAGAAACAATGCACATGGATGAGAATACCAACCTTGCAATGTCATCGCCAA
TTTCTGGTGTGATCCTAAAACTTCGCCGTCTAGTTTTACGAATTTCTGATGTGGATGAAC
TGTTTGAAAGCTAGAGAATATTAAAAAATTGCTTGCACAGCCAATCAAATATATACATGC
AGACTTGTATAAGCAACTTAACTAATACTGGAATGTATATGATAGGAGCGGCATACTGAA
AATTTTCGCATGCACTAATGTAAAAATGAGTTACCTTGAAGGAGATGATGCTTCACCAGT
GTAATCCCTAAAAGAATTCCTACGGNTGCTCA

Last Updated on 06.12.2018 16:11