Sequences from confirmation amplicon


Confirmation sequences for line 026A09 and hit At2g30670 are shown.
Line ID 026A09
Locus At2g30670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13069984
Primers used for PCR 09oi - 8474, show primer details
   
Sequence Name 54-K114722-0022-026-A09C-09oi
GenBank Accession LN508565 [GenBank]
Sequencing primer gene specific primer, 09oi: 5'-GGCGTCTTTGAAAAACTGATTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K114722-0022-026-A09C-09oi
ATAAAATTAGGAGCAACAAATTTGGCTCTTATGCCATCTCTTGCCCATTCACATGCCAAA
TTTTTTGCTATTTGATTCAAAGCTCCGGCTCATACAAGCTTCAAAAACGATAACCTACAA
AACATCTTTTTTTAAGGTTCAAAAATACTAATTAAACCTTTATTGAAATGGTTGCATTAC
CCTATAAAAACAATCATTTACCGTTGGAAATACTTTCAAGTAAAACTATGTTTATCCTTC
AAAATAATTAAAATACCTTTAAATTATTCTTGTTTGCGTATTTATAATTGTTACTGAATA
TAAATGAAAACCCTTATTTTTTTACACAAATTTAAACAAAAATTATTTACTTTTACAAAT
GTAAAAAAACTCAAATTTAATTTACCAAATGGCTTCATGTCCGGGAAATCTACTTGGATC
ACCATTGAGTATAATGGTCAATATGGAAAAAAAAAAAAAGTATTTACCATTTTTTTTTCA
TTTCAAAAATGTAAA
   
Sequence Name 42-K114721-0022-026-A09C-8409
GenBank Accession LN504562 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114721-0022-026-A09C-8409
ACGTAAATACTTTTTGTTTAAATCTGTGTAAAAATATAAGGGTTTTCATTCATATTCAGT
AACAATTATAAATACGCAAACAAGACTAATTTAAAGCTATTTTAATTATCTTGATGGATA
ACTATAGTCTTACTTGAAAGTATTTCCAACGGTAACTGATTGTTCTAATAGGCTAATGCA
ACCATTTCAATAAAGGTCTAATTAGTATCTTTGAACCTAATATATAGATGTCTCGTAGTT
TATCGTTTTTGATGCTTGTATGAGCAGGAGCTTTGAATCAACTAGCAAAAACTTTGGCAT
GTGAATGGGCAAGAGATGGCATAAGAGCCAACTCTGTTGCTCCTAATTTTATCTACACTG
CTATGGCTCAACCTGTACGTAATCATCAATGAGTACACTCTGGCTTCTTCTTCTTTTTTT
TTTTCCAATTGACATCAAACACTTCTCATTCTAATGTCTTTAAATCTGGTGAATAATCAT
TT

Last Updated on 06.12.2018 16:11