Sequences from confirmation amplicon


Confirmation sequences for line 026E11 and hit At5g48100 are shown.
Line ID 026E11
Locus At5g48100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19492244
Primers used for PCR 01pd - 8474, show primer details
   
Sequence Name 28-K115495-0022-026-E11C-01pd
GenBank Accession LN498980 [GenBank]
Sequencing primer gene specific primer, 01pd: 5'-ATTAGTCAAAACGGACCAAGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K115495-0022-026-E11C-01pd
TTTTGTAATACAAAATTCTTATATCCGAATGTAACTAATTTGAGAAAGACGGTCTCTATA
CGTATATAAAATATATGTATATATGCCTCTCTATTCTTTGATGAACTTCAGTCAGGGTAA
TTAATTTACTAAAACTCTTCCAAAATGTCACATTCCTTCTTCAATTTATTCCTAATCTCT
CTTTTCCTCTACAACAATTGCATTGCGCATCACTATACATTCACGGTAAGTTTAAATTAG
TTAGTCAATTGGGCGTGAGTAAACAAACTTTAATTGATTAAAACTAATAAAATCGTTAAT
TAATGGCAGGTTAGGGAAGTTCCATATACGAAACTGTGTAGCACGAAGGCGATTTTAACC
GTTAATAGTCAGTTTCCTGGACCGATTATAAAGGTTCACAAAGGAGACACTATTTATGTT
AACGTTCAAAACCGAGCTAGTGAAAACATCACAATGCATTGGTAGTCTAAAGACCATTTG
TATTATACCACTTAAGACGATGATGATTGAATAAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 17-K115494-0022-026-E11C-8409
GenBank Accession LN493987 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115494-0022-026-E11C-8409
ATATACAAATGGTCTTTGACTACCAATGCATTGTGATGTTTTCACTAGCTCGGTTTTGAA
CGTTAACATAAATAGTGTCTCCTTTGTGAACCTTTATAATCGGTCCAGGAAACTGACTAT
TAACGGTTAAAATCGCCTTCGTGCTACACAGTTTCGTATATGGAACTTCCCTAACCTGCC
ATTAATTAACGATTTTATTAGTTTTAATCAATTAAAGTTTGTTTACTCACGCCCAATTGA
CTAACTAATTTAAACTTACCGTGAATGTATAGTGATGCGCAATGCAATTGTTGTAGAGGA
AAAGAGAGATTAGGAATAAATTGAAGAAGGAATGTGACATTTTGGAAGAGTTTTAGTAAA
TTAATTACCCTGACTGAAGTTCATCAAAGAATAGAGAGGCATATATACATATATTTTATA
TACGTATAGAGACCGTCTTTCTCAAATTAGTTACATTCGGATATAAGAATTTTGTATTTT
ACAAAATATGTTTTCGGTAAGCATCATCTTCTTGGTCCGTTTTGACTAA
Primers used for PCR 08pl - 8474, show primer details
   
Sequence Name 29-K115550-0022-026-E11C-08pl
GenBank Accession LN499427 [GenBank]
Sequencing primer gene specific primer, 08pl: 5'-AAGAAGATGATGCTTACCGAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K115550-0022-026-E11C-08pl
CTAATTTGAGAAAACGGTCTCTATACGTATATAAAATATATGTATATATGCCTCTCTATT
CTTTGATGAACTTCAGTCAGGGTAATTAATTTACTAAAACTCTTCCAAAATGTCACATTC
CTTCTTCAATTTATTCCTAATCTCTCTTTTCCTCTACAACAATTGCATTGCGCATCACTA
TACATTCACGGTAAGTTTAAATTAGTTAGTCAATTGGGCGTGAGTAAACAAACTTTAATT
GATTAAAACTAATAAAATCGTTAATTAATGGCAGGTTAGGGAAGTTCCATATACGAAACT
GTGTAGCACGAAGGCGATTTTAACCGTTAATAGTCAGTTTCCTGGACCGATTATAAAGGT
TCACAAAGGAGACACTATTTATGTTAACGTTCAAAACCGAGCTAGTGAAAACATCACAAT
GCATTGGTAGTCTAAAGACCATTTGTATTATACCACTTAAGACGATGATGATTGAATAAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA
   
Sequence Name 02-K115549-0022-026-E11C-8409
GenBank Accession LN487167 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115549-0022-026-E11C-8409
ATACAAATGGTCTTTGACTACCAATGCATTGTGATGTTTTCACTAGCTCGGTTTTGAACG
TTAACATAAATAGTGTCTCCTTTGTGAACCTTTATAATCGGTCCAGGAAACTGACTATTA
ACGGTTAAAATCGCCTTCGTGCTACACAGTTTCGTATATGGAACTTCCCTAACCTGCCAT
TAATTAACGATTTTATTAGTTTTAATCAATTAAAGTTTGTTTACTCACGCCCAATTGACT
AACTAATTTAAACTTACCGTGAATGTATAGTGATGCGCAATGCAATTGTTGTAGAGGAAA
AGAGAGATTAGGAATAAATTGAAGAAGGAATGTGACATTTTGGAAGAGTTTTAGTAAATT
AATTACCCTGACTGAAGTTCATCAAAGAATAGAGAGGCATATATACATATATTTTATATA
CGTATAGAGACCGTCTTTCTCAAATTAGTTACATTCGGATATAAGAATTTTGTATTTTAC
AAAATATGTTTTCGGTAAGCATCATCTTCT

Last Updated on 06.12.2018 16:11