Sequences from confirmation amplicon


Confirmation sequences for line 026G05 and hit At1g14910 are shown.
Line ID 026G05
Locus At1g14910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5141770
Primers used for PCR 04hj - 8474, show primer details
   
Sequence Name 51-K112479-0022-026-G05C-04hj
GenBank Accession LN507549 [GenBank]
Sequencing primer gene specific primer, 04hj: 5'-TGGTACATTAAACTAAACTCGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K112479-0022-026-G05C-04hj
GTCAATATATACTATCTGGAAAGAACANCTTGATTTAAGCTCTGAGATCTTATTGTAAAT
TGTGTATGCAGGTATTGAAGGAGAGTTTTAAAGTGTATTGTGCCATCAATGAAGGAATTA
TCAATCTAGTAGAGAAGGTAGGCCAGTTTTTTCTTTTCCCCCAGAGTTACTCTCTCCCTT
CATGTGTTTTTTGATTTCTTACTGTTTTATACATGCATATATTGCAGTTCTTTGAAATGC
CAAGACATGAGGCCATCAAGGCCCTTGAAATATACAAGCGTGCGGGTTTGCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTA
   
Sequence Name 21-K112478-0022-026-G05C-8409
GenBank Accession LN495728 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112478-0022-026-G05C-8409
ATTTCATTGAATATATCCTGCAACCCGCACGCTTGTATATTTCAAGGGCCTTGATGGCCT
CATGTCTTGGCATTTCAAAGAACTGCAATATATGCATGTATAAAACAGTAAGAAATCAAA
AAACACATGAAGGGAGAGAGTAACTCTGGGGGAAAAGAAAAAACTGGCCTACCTTCTCTA
CTAGATTGATAATTCCTTCATTGATGGCACAATACACTTTAAAACTCTCCTTCAATACCT
GCATACACAATTTACAATAAGATCTCAGAGCTTAAATCAAGCTGTTCTTTCCAGATAGTA
TATATTTGACACAACACTAAGAACTAGCGGATATATGTAAATTATGCCCCCGAGTTTAGT
TTAATGTACC

Last Updated on 06.12.2018 16:11