Sequences from confirmation amplicon


Confirmation sequences for line 027B09 and hit At5g27200 are shown.
Line ID 027B09
Locus At5g27200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9571552
Primers used for PCR 02et - 8474, show primer details
   
Sequence Name 29-K110907-0022-027-B09C-02et
GenBank Accession LN499341 [GenBank]
Sequencing primer gene specific primer, 02et: 5'-CATCACTATCTCAACCTGCAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K110907-0022-027-B09C-02et
ATGTCATGAGCTCTGAGATACAATACAATGAGATGATATTTACCGTGTCGAGACAATCAG
CTCCAAGCTCGGTAAATTTAGTTCCGGCAGTGACTTTTTGATCATCGGTGAGCGACAATT
GCTTCTTGACAATCTCAGACACTTTCTCTACTGTCTCTTGCTTGACCTTAAACCACATAT
ATACCACATTACCAAATATATTCAAACAGCCTCAAGAAAGTCGAAGAAATATAGAGTTAA
ATCAACATACTGCACAAGAAACTGCGAGACGAGCAATGTTTTGCACAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 13-K110898-0022-027-B09C-8409
GenBank Accession LN492093 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K110898-0022-027-B09C-8409
TTGAATATATCCTGTGCAAACATTGCTCGTCTCGCAGTCTCTTGTGCAGTATGTTGATTT
AACTCTATATTTCTTCGACTTTCTTGAGGCTGTTTGAATATATTTGGTAATGTGGTATAT
ATGTGGTTTAAGGTCAAGCAAGAGACAGTAGAGAAAGTGTCTGAGATTGTCAAGAAGCAA
TTGTCGCTCACCGATGATCAAAAAGTCACTGCCGGAACTAAATTTACCGAGCTTGGAGCT
GATTCTCTCGACACGGTAAATATCATCTCATTGTATTGTATCTCAGAGCTCATTGGACAT
CATCATCAACAAATTCTGCTTTCTTTTTGCAGGTTGAGATAGTGATG

Last Updated on 06.12.2018 16:11