Sequences from confirmation amplicon


Confirmation sequences for line 027C03 and hit At1g73160 are shown.
Line ID 027C03
Locus At1g73160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27507074
Primers used for PCR 01jr - 8474, show primer details
   
Sequence Name 70-K113261-0022-027-C03C-01jr
GenBank Accession LN512421 [GenBank]
Sequencing primer gene specific primer, 01jr: 5'-GGCTCTAAAGCTCCTAAAACCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K113261-0022-027-C03C-01jr
ATCCAGCCACAAGCAAATAGACTTTCGGGTGCATTTTAACGAGCAAAGCAAACGCTTCAT
ATAGAAGTGGATGTCCTTTGTCTCTCACCAAACGTCCAGACACACCCATCACAATATATG
TCCCATTGTCAGGAATACCGTGTTTCGCCCTGAACCTAGCTCCAGATTCAGGAGAATACA
CGAACTTGGTTTGGTCAACACCGTTGACTATAACGTGAACTTTTCTCTTAGGAAGTTGAT
AGATGTTAACTAGTACCTCGCGCGCGCTGTTGCTAATGCAAATATGCTGTTTATATTTTG
GGAAGAATCTGATTTCATCGACGAGTCTAGGCATCGTTTGTTGAAGATCAGAGCTAGGAC
GATCATTGGAAAGTTCTTGAAAAAGATTTGAATGCATAATCTCGTACCAGATTCCGTGCC
ATGTCACAGCTATGTCACCATTAGGCACCATTTTGACTCGCCAATGAGGCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCACGGTTATTA
   
Sequence Name 51-K113260-0022-027-C03C-8409
GenBank Accession LN507563 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113260-0022-027-C03C-8409
TCATTGAATATATCCTGCCTCATTGGCGAGTCAAAATGGTGCCTAATGGTGACATAGCTG
TGACATGGCACGGAATCTGGTACGAGATTATGCATTCAAATCTTTTTCAAGAACTTTCCA
ATGATCGTCCTAGCTCTGATCTTCAACAAACGATGCCTAGACTCGTCGATGAAATCAGAT
TCTTCCCAAAATATAAACAGCATATTTGCATTAGCAACAGCGCGCGCGAGGTACTAGTTA
ACATCTATCAACTTCCTAAGAGAAAAGTTCACGTTATAGTCAACGGTGTTGACCAAACCA
AGTTCGTGTATTCTCCTGAATCTGGAGCTAGGTTCAGGGCGAAACACGGTATTCCTGACA
ATGGGACATATATTGTGATGGGTGTGTCTGGACGTTTGGTGAGAGACAAAGGACATCCAC
TTCTATATGAAGCGTTTGCTTTGCTCGTTAAAATGCACCCGAAAGTCTATTTGCTTGTGG
CTGGATCTGGTCCTTGGGGCAAAAGATACGCGGAATTAGGCGAAAACGTTAGGGTTTTAG
GAGCTTTAGAGC

Last Updated on 06.12.2018 16:11