Sequences from confirmation amplicon


Confirmation sequences for line 027F09 and hit At1g22050 are shown.
Line ID 027F09
Locus At1g22050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7772238
Primers used for PCR CV07 - 8474, show primer details
   
Sequence Name 06-K030752-022-027-F09C-8409
GenBank Accession LN488685 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K030752-022-027-F09C-8409
ATTTACAATTGAACTTTGTAGTCTTTGGGCTATACTGATCATTTGGGCTTTAATAGATAA
TTATAGTTTTATAGTGTGAAAGGTTTAGTTGTTGTTGTGTTAAGATCACTCTAGTTACTG
ATTTTGGCTAAAACTAGTATAAGATTGATGTCTCAAATTGTATTGTTTTTGCCTGGATTT
TTTTGGTTTAGGTGTTTGGTGTTTCTTGGCCTTATTGAATCTTATGTTGCATTTACTTTC
TCTACTTAAGGTTGTTTTATAATTGCATTTTCAATGTTTCTCTGTTTCACAGATAAAGAA
AATACTCCAAAAACTGTAAATGATATGAAGCTCATCAATGCCGGGAAAATACTGGAGAAC
AATAGGACACTTGCTGAATCCAGGCTTCCTGTTTGTGAACTTCCTGGAATGATCATTACT
ATGCATATTGTTCTTCGCCTTCCTACGTTAGACAAAAAGAGCGGTAATTTTCTCTACCTT
GTTATACTGTTTATCGCAGTGTAACAACATGTTCCCTTGAATTCTGTACTCGTTAACTAT
GGCTTTGAGATCATGTTCATATCAAAGCNNTNGTTNACGAGTACAGAATTCNAGGGAACA
TGTTGTTACACTGCGATAAACAGTATAACAAGGTAGAG
   
Sequence Name 45-K030752-022-027-F09C-CV07
GenBank Accession LN505420 [GenBank]
Sequencing primer gene specific primer, CV07: 5'-TGATATGAACATGATCTCAAAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K030752-022-027-F09C-CV07
TCAGGGACTGTTGTTACCTGCGATAACAGTATAACAAGGTAGAGAAAATTACCGCTCTTT
TTGTCTAACGTAGGAAGGCGAAGAACAATATGCATAGTAATGATCATTCCAGGAAGTTCA
CAAACAGGAAGCCTGGATTCAGCAAGTGTCCTATTGTTCTCCAGTATTTTCCCGGCATTG
ATGAGCTTCATATCATTTACAGTTTTTGGAGTATTTTCTTTATCTGTGAAACAGAGAAAC
ATTGAAAATGCAATTATAAAACAACCTTAAGTAGAGAAAGTAAATGCAACATAAGATTCA
ATAAGGCCAAGAAACACCAAACACCTAAACCAAAAAAATCCAGGCAAAAACAATACAATT
TGAGACATCAATCTTATACTAGTTTTAGCCAAAATCAGTAACTAGAGTGATCTTAACACA
ACAACAACTAAACCTTTCACACTATAAAACTATAATTATCTATTAAAGCCCAAATGATCA
GTATAGCCCAAAGACTACAAAGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11