Sequences from confirmation amplicon


Confirmation sequences for line 027G10 and hit At5g03260 are shown.
Line ID 027G10
Locus At5g03260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:777400
Primers used for PCR KC76 - 8474, show primer details
   
Sequence Name 02-K103100-0022-027-G10C-KC76
GenBank Accession LN486987 [GenBank]
Sequencing primer gene specific primer, KC76: 5'-ATTTCATTTCTTATGCAATTTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K103100-0022-027-G10C-KC76
AACATCTCAGACACAAATCAACAGAAGGGAAATACATCAACAAAGAATATCATCAGCGAA
ACTTAACAAATAAAGACGAAGAATCAGAGTAGTGTTCCCATCTCAGATTCTTAACAGGAC
GGATAGTCTTTAGGCGGTGGTAGAACGGAAAGCTCAGGTGTTTCCCCGTTCTCGACGACG
AAAGCCATCTTCAAACCCCACATTGTATGGACTTCCAAGTGACAGTGCATAAACCAAACA
CCTGCAACAACACATGCATTGATCAGTTGATCTACTTATCATATATGGTCATCATCATAA
TCATATATGTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 02-K103101-0022-027-G10C-8409
GenBank Accession LN486988 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K103101-0022-027-G10C-8409
GCCATTTACAATTGAATATATCCTGCACATATATGATTATGATGATGACCATATATGATA
AGTAGATCAACTGATCAATGCATGTGTTGTTGCAGGTGTTTGGTTTATGCACTGTCACTT
GGAAGTCCATACAATGTGGGGTTTGAAGATGGCTTTCGTCGTCGAGAACGGGGAAACACC
TGAGCTTTCCGTTCTACCACCGCCTAAAGACTATCCGTCCTGTTAAGAATCTGAGATGGG
AACACTACTCTGATTCTTCGTCTTTATTTGTTAAGTTTCGCTGATGATATTCTTTGTTGA
TTGTATTTCCCTTCTGTTGATTTGTGTCTGAGATTGTTTCCTATGGGGCTGCATAACATA
ATACACCCCGAAATTGCATAAGAAATGAAA

Last Updated on 06.12.2018 16:11