Sequences from confirmation amplicon


Confirmation sequences for line 027H10 and hit At4g37590 are shown.
Line ID 027H10
Locus At4g37590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17663598
Primers used for PCR DC71 - 8474, show primer details
   
Sequence Name 66-K032737-022-027-H10C-DC71
GenBank Accession LN511528 [GenBank]
Sequencing primer gene specific primer, DC71: 5'-TGTTGAGTTGATGCAGAGCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K032737-022-027-H10C-DC71
ATGTAACCGCAAAAGCATCAGTTGCGAAACTTGTTGATGGTTATTTAGCTGAGAAATCGA
GAGATTCCGATAATTTGCCTCTTCAGAAGTTCCTCTCACTTGCGGAAATGGTCTCGAGCT
TTCCTAGACAGTCTCACGATGGAGTTTATCGCGCTATCGACATGTTTCTTAAGGTACAAG
ATCTTATTTATCGCGCTATCGACATGTTTCCTAGACATGTTTCCTAGACAGTCTCACGAT
GGAGTTTATCGCGCTATCGACATGTTTCTTAAGCTCTATCTCAACAAAGTATTCATACAA
AGATCTTATTTAGCAATACCTAATCCGTTGTTTCGGTCAGTTGTTAGTAATCAAGGTTTG
ATCAAGTAACTAATCAAGATTTTGATTTCTTGATTTTGATTTTGATTTTGATTAGTAATC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTCA
   
Sequence Name 34-K032737-022-027-H10C-8409
GenBank Accession LN501309 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K032737-022-027-H10C-8409
GAGCCTTTACAATTGAATATATCCTGATTACTAATCAAAATCAAAATCAAAATCAAGAAA
TCAAAATCTTGATTAGTTACTTGATCAAACCTTGATTACTAACAACTGACCGAAACAACG
GATTAGGTATTGCTAAATAAGATCTTTGTATGAATACTTTGTTGAGATAGAGCTTAAGAA
ACATGTCGATAGCGCGATAAACTCCATCGTGAGACTGTCTAGGAAACATGTCTAGGAAAC
ATGTCGATAGCGCGATAAATAAGATCTTGTACCTTAAGAAACATGTCGATAGCGCGATAA
ACTCCATCGTGAGACTGTCTAGGAAAGCTCGAGACCATTTCCGCAAGTGAGAGGAACTTC
TGAAGAGGCAAATTATCGGAATCTCTCGATTTCTCAGCTAAATAACCATCAACAAGTTTC
GCAACTGATGCTTTTGCGGTTACATCATCCTCTCTCGGGTCTCGGGATTTCAAGAAAACC
TCAACTAAGCTCTG

Last Updated on 06.12.2018 16:11