Sequences from confirmation amplicon


Confirmation sequences for line 028F09 and hit At3g28415 are shown.
Line ID 028F09
Locus At3g28415 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10649789
Primers used for PCR CM74 - 8474, show primer details
   
Sequence Name 01-K104111-0022-028-F09C-CM74
GenBank Accession LN486550 [GenBank]
Sequencing primer gene specific primer, CM74: 5'-TCAACATCTCGAGAAAAATACGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104111-0022-028-F09C-CM74
AGCCATCTCGTTGGTGCGAACCGTCTACGCATTTGGTAGTGCGATAAAGATGATATCGAA
ATTCTCAGCTGCGCTTGAAGGCTCATTGAAGCTAGGGTTGAGACAAGGAATAGCTAAAGG
AATCGCCATTGGGAGCAATGGTGTCACTTACGCCATTTGGGGGTTCATGACTTGGTACGG
AAGTCGGATGGTTATGTACCATGGAGCTAAAGGCGGTACTATCTTCGCAGTCATCATATG
CATCACCTATGGTGGCACGTAAGTATATATACATGTCTAAGGCTCTTTGGTATTCTTATT
ATAGTTTTGATATTTTGTATCTTACGTAGATCACTTGGTCGAGGTTTGTCGAATCTCAAA
TATTTCTCCGAGGCGGTTGTAGCAGGGGAAAGGATTATTGAAGTGATAAAAAGAGTTCCC
GATATTGATTCGGACAACCCGAGAGGTCAAGTTCTTGAGAATATTAAAGGAGAAGTCCAG
TTTAAGCATGTGAAGTTCATGTACTCATCAAGACCCGAGACACCAATCTTTGATGATTTA
TGTCTAA
   
Sequence Name 17-K104112-0022-028-F09C-8409
GenBank Accession LN493826 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K104112-0022-028-F09C-8409
ATTTCTTGCTAATTCTTGTAAAGCGATATCACAGTTGATTTTCCTGATCCGCTTCCACCC
ACCAGAGCAACAGATTTACCAGATGGGATTCTTAGACATAAATCATCAAAGATTGGTGTC
TCGGGTCTTGATGAGTACATGAACTTCACATGCTTAAACTGGACTTCTCCTTTAATATTC
TCAAGAACTTGACCTCTCGGGTTGTCCGAATCAATATCGGGAACTCTTTTTATCACTTCA
ATAATCCTTTCCCCTGCTACAACCGCCTCGGAGAAATATTTGAGATTCGACAAACCTCGA
CCAAGTGATCTACGTAAGATACAAAAATATCAAACTATAATAAGAATACCAAAGAGCCTT
AGACATGTATATATACTTACGTGCCACCATAAGTGATG

Last Updated on 06.12.2018 16:11