Sequences from confirmation amplicon


Confirmation sequences for line 030C11 and hit At4g04460 are shown.
Line ID 030C11
Locus At4g04460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2225398
Primers used for PCR JL01 - 8474, show primer details
   
Sequence Name 01-K027644-022-030-C11C-8409
GenBank Accession LN486434 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K027644-022-030-C11C-8409
TAGATTTCCCGGACATGAAGCCATTTACCGAGGGTCTCATTGGTCTCCCAAACACTATTT
TCGCCTGAACGATGAAAATGCGGATATGGTTCCGCTGAAAAACTATTTGGATGCTCAATA
CTATGGTGACATTACCATTGGTACTCCTCCTCAGAAGTTCACTGTGATCTTTGATACTGG
TAGCTCCAATCTCTGGATACCATCTACTAAATGTTACCTATCGGTACGCGTTAGTTTGTT
CTTAATGCATAATAATTCTGAAAATCCTATTGTATTCTTACTCCCAAAAGAACAAAACAA
AATATCTATGTTTAATGTTTTTTCAGGTTGCTTGTTATTTCCACTCCAAGTACAAGGCTA
GCCAGTCATCGTCATATAGAAAGAACGGTTCGTTCTCTCTGTTCATCTTGTTATTTGATA
CTCGAGCTTCGGTACTTTGATGTTTCTTGATAACTATTCAAGAAAGGGATACATTTAGCG
CCGGTCTTTTGATATGTTTGCTGATAGGTT
   
Sequence Name 05-K027644-022-030-C11C-JL01
GenBank Accession LN488212 [GenBank]
Sequencing primer gene specific primer, JL01: 5'-TTTTATCGTCAAAACCTATCAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K027644-022-030-C11C-JL01
ACATATCTCAAGACCGGCGCTAAATGTATCCCTTTCTTGAATAGTTATCAAGAAACATCA
AAGTACCGAAGCTCGAGTATCAAATAACAAGATGAACAGAGAGAACGAACCGTTCTTTCT
ATATGACGATGACTGGCTAGCCTTGTACTTGGAGTGGAAATAACAAGCAACCTGAAAAAA
CATTAAACATAGATATTTTGTTTTGTTCTTTTGGGAGTAAGAATACAATAGGATTTTCAG
AATTATTATGCATTAAGAACAAACTAACGCGTACCGATAGGTAACATTTAGTAGATGGTA
TCCAGAGATTGGAGCTACCAGTATCAAAGATCACAGTGAACTTCTGAGGAGGAGTACCAA
TGGTAATGTCACCATAGTATTGAGCATCCAAATAGTTTTTCAGCGGAACCATATCCGCAT
TTTCATCGTTCAGGCGAAAATAGTGTTTGGGAGACCAATGAGACCCTCGGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11