Sequences from confirmation amplicon


Confirmation sequences for line 030D05 and hit At1g20270 are shown.
Line ID 030D05
Locus At1g20270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7022582
Primers used for PCR 04co - 8474, show primer details
   
Sequence Name 31-K108475-0022-030-D05C-04co
GenBank Accession LN500197 [GenBank]
Sequencing primer gene specific primer, 04co: 5'-CCATTGGTTGATCTCATGTGTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K108475-0022-030-D05C-04co
AACCATTCCCAACAATAAAATGACGAAATCATCCCTATTTTCTCAACCACCGACCACCGT
AAATATCGCCACATCATCACGTAAATAAGGGTACAAATGTCATTTACAGATGCAGCGTTA
GAACAGAACAGAGATTAAAGAAAAAAGACAGAGAAAGAATCCTTATTAGAAGGAAAAATC
ATTTATGAGTGAAATAGAAATTTGATATCTCTTTATTAGTTTCTGCATCTCGAGGGGTTT
TTGGGATTCTCTGATTCGATCGAGTTCTATTTGTCGTTGTGTACAACATCTCGGGTGGAT
CTTTGTTGATGGCGAAACTGAGACACTCGCGATTTCAAGCGAGGAAATGGTCGACATTGA
TGCTGGTTCTGTTTATGTTGTTTATGTTAACGATTGTTCTCTTAATGCTTTTGGCTTTCG
GAGTTTTCTCTCTTCCTATTAACAACGATGAATCCTCTCCTATCGATCTCTCTTATTTCC
GACGAGCTGCCACTGAGAGGTGAGCTTTGCATGAATTTCTTGATCTCTCTGGAATTTTTA
TATTAAATTTGTTTAATTTCCTTTTTGTTTGTGTATCGAATTCAGAAGTGAAGGACTTGG
GAAGAGAGGAGACCAGTGGACTGAAGTGCTTTCTTGGGAACCTAGAGCTTTTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGGGTTATT
   
Sequence Name 15-K108474-0022-030-D05C-8409
GenBank Accession LN493008 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K108474-0022-030-D05C-8409
AAGCTCTAGGTTCCCAAGAAAGCACTTCAGTCCACTGGTCTCCTCTCTTCCCAAGTCCTT
CACTTCTGAATTCGATACACAAACAAAAAGGAAATTAAACAAATTTAATATAAAAATTCC
AGAGAGATCAAGAAATTCATGCAAAGCTCACCTCTCAGTGGCAGCTCGTCGGAAATAAGA
GAGATCGATAGGAGAGGATTCATCGTTGTTAATAGGAAGAGAGAAAACTCCGAAAGCCAA
AAGCATTAAGAGAACAATCGTTAACATAAACAACATAAACAGAACCAGCATCAATGTCGA
CCATTTCCTCGCTTGAAATCGCGAGTGTCTCAGTTTCGCCATCAACAAAGATCCACCCGA
GATGTTGTACACAACGACAAATAGAACTCGATCGAATCAGAGAATCCCAAAAACCCCTCG
AGATGCAGAAACTAATAAAGAGATATCAAATTTCTATTTCACTCATAAATGATTTTTCCT
TCTAATAAGGATTCTTTCTCTGTCTTTTTTCTTTAATCTCTGTTCTGTTCTAACGCTGCA
TCTGTAAATGACATTTGTACCCTTATTTACGTGATGATGTGGCGATATTTACGGTGGTCG
GTGGTTGAGAAAATAGGGATGATTTCGTCATTTTATTGTTGGGAATTGGTTTTGTTTCCT
AGCACAATGTTTTAGTTATTACACATGAGATCAACCAATGG

Last Updated on 06.12.2018 16:11