Sequences from confirmation amplicon


Confirmation sequences for line 030F06 and hit At2g02680 are shown.
Line ID 030F06
Locus At2g02680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:748369
Primers used for PCR 07ne - 8474, show primer details
   
Sequence Name 32-K114376-0022-030-F06C-07ne
GenBank Accession LN500679 [GenBank]
Sequencing primer gene specific primer, 07ne: 5'-TCTTATTGTTCGTTGATGAGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K114376-0022-030-F06C-07ne
TTAATTTGAATTNAAAATATAAAATTTTATTAGTAGATATTCATATGCCTAGTTGATCCA
GTTGACTCTTCCTGTTTCATAATACTAGTATCCACCCTCTTCTCAAACGGGATTGTTAAA
ATCTTATACATTGCATTATATGTAGCCTTTACACTAACGTTTTACTTGATTTTTCTTTTG
GTTTGGTTATCGATCGATGATCCTGATACAAAAAATTACAACTATAAAAGTTTATTTTCT
TTTTGGTTCGGTTTCAATAATTATTTTGGTTTGGTTATGATCACCTCAATTGGAATGGAC
ATCTCAACATGTGCATTGTGAGTGAGAATTTGTCTTGAAAAATAAAAGACCTTTTTTTAC
AAAAATTTGTAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCACAATTATT
   
Sequence Name 18-K114375-0022-030-F06C-8409
GenBank Accession LN494404 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114375-0022-030-F06C-8409
CATTACAATTTTTGTAAAAAAGGTCTTTTATTTTTCAGACAAATTCTCACTCACAATGCA
CATGTTGAGATGTCCATTCCAATTGAGGTGATCATAACCAAACCAAAATAATTATTGAAA
CCGAACCAAAAAGAAAATAAACTTTTATAGTTGTAATTTTTTGTATCAGGATCATCGATC
GATAACCAAACCAAAAGAAAAATCAAGTAAAACGTTAGTGTAAAGGCTACATATAATGCA
ATGTATAAGATTTTAACAATCCCGTTTGAGAAGAGGGTGGATACTAGTATTATGAAACAG
GAAGAGTCAACTGGATCAACTAGGCATATGAATATCTACTAATAAAATTTTATATTTTAC
AATTCAAATTTAATGGTCCATGTGCCATATATAATATAATTGATCTGACGATCTCATCAA
CGAACAATAAG

Last Updated on 06.12.2018 16:11