Sequences from confirmation amplicon


Confirmation sequences for line 031B04 and hit At1g03410 are shown.
Line ID 031B04
Locus At1g03410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:845761
Primers used for PCR KC03 - 8474, show primer details
   
Sequence Name 54-K035533-022-031-B04C-KC03
GenBank Accession LN508399 [GenBank]
Sequencing primer gene specific primer, KC03: 5'-AACATGATATGAGACTTGGCACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K035533-022-031-B04C-KC03
AGCCTCACATAGAAGCTCAAAGAGAAATTCACCTAAAGTCATTAGTTGCTTTGAGTACTC
CATCATAATCTCCCTGTCACATCAAAATATTTGCAAGAGATTAGCGTACGTCTAGCTATT
CACAAGGCACATATAACAAATCTATTCAAAATAACTTTGGTATTTAACTTTTTGATTTAT
TTATCTTTAATTTAATATATACATTGAAAGATCTGTTAAAATCCAATGTGAAATAAATAA
ATAATTTATTAAAAACCAATGAAATCATTAAAAGGTTATAATTACTATTTAGAAATCAAT
AACTCATGATAAAATCAAGTGTAGAATCCACTAACTTATGTAGTTATTAATTATTGACTT
TTTATGCTTTTTCAAGTTTAAATTTTGTAATAACCGAGTGTTTACCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCC
AATGG
   
Sequence Name 06-K035533-022-031-B04C-8409
GenBank Accession LN488713 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K035533-022-031-B04C-8409
TGAAGCCTTTACAATTGGTAAACACTCGCGTTATTACAAAATTTAAACTTGAAAAAGCAT
AAAAAGTCAATAATTAATAACTACATAAGTTAGTGGATTCTACACTTGATTTTATCATGA
GTTATTGATTTCTAAATAGTAATTATAACCTTTTAATGATTTCATTGGTTTTTAATAAAT
TATTTATTTATTTCACATTGGATTTTAACAGATCTTTCAATGTATATATTAAATTAAAGA
TAAATAAATCAAAAAGTTAAATACCAAAGTTATTTTGAATAGATTTGTTATATGTGCCTT
GTGAATAGCTAGACGTACGCTAATCTCTTGCAAATATTTTGATGTGACAGGGAGATTATG
ATGGAGTACTCAAAGCAACTAATGACTTTAGGTGAATTTCTCTTTGAGCTTCTATCTGAG
GCTTTGGGATTAAACCCTAATCACCTCAAGGACATGGGCTGTGCCAAGTCTCATATCA

Last Updated on 06.12.2018 16:11