Sequences from confirmation amplicon


Confirmation sequences for line 031D07 and hit At1g26320 are shown.
Line ID 031D07
Locus At1g26320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9105605
Primers used for PCR 10jx - 8474, show primer details
   
Sequence Name 62-K113261-0022-031-D07C-10jx
GenBank Accession LN510803 [GenBank]
Sequencing primer gene specific primer, 10jx: 5'-AAAACATCACATAGCCAAGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K113261-0022-031-D07C-10jx
ATAGATATTATACCAAGAAGTCCAGTGTAGTAAGATAATGGAACATCGGTATGTTCGATT
TTGAAGTGTGAGTAAGTAGTTAAAGTAATAACACTGTACTCCTCCCATCCAACTAGTCCC
CAAAGTAAGTCTCCCTTTTTGTAATCTGGATGTCCTGAATCAATCACTTTAGACACTCCA
AGCCCAATGATTGGCTGCATAGACAACATCTTTAACACGAAGTATACAAGAACAAAATAT
CCCGAAGAAATGTTCAATTTTGCTAGAGGTATAATTTCTTCGAGAACACAATATTCACCA
GAAATGTTGCAATTAAAACAATTTATCCGTAATGAACATTAATTGTCTAACATGCACATC
CGTTTCATATTAGAATCTCTTGTTGGATTCTTAGCAGCATTATCGAAAATTAAATTCATA
CGAGACAACCCTACATACTGTCATACACACGGATAATAATATTTCTTAAACGAGATATAA
ATTTTAAAATCCATCATTGGTTCCAAAACATTTGGAGAAATCTACATGGATCAGCAATAA
ACACCATGGTGACAAAGATTCAAATTAAACACTGCTATCCGGGAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 43-K113260-0022-031-D07C-8409
GenBank Accession LN504902 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113260-0022-031-D07C-8409
TTTCCCGGATAGCAGTGTTTATTTGAATCTTTGTCACCATGGTGTTTATTGCTGATCCAT
GTAGATTTCTCCAAATGTTTTGGAACCAATGATGGATTTTAAAATTTATATCTCGTTTAA
GAAATATTATTATCCGTGTGTATGACAGTATGTAGGGTTGTCTCGTATGAATTTAATTTT
CGATAATGCTGCTAAGAATCCAACAAGAGATTCTAATATGAAACGGATGTGCATGTTAGA
CAATTAATGTTCATTACGGATAAATTGTTTTAATTGCAACATTTCTGGTGAATATTGTGT
TCTCGAAGAAATTATACCTCTAGCAAAATTGAACATTTCTTCGGGATATTTTGTTCTTGT
ATACTTCGTGTTAAAGATGTTGTCTATGCAGCCAATCATTGGGCTTGGAGTGTCTAAAGT
GATTGATTCAGGACATCCAGATTACAAAAAGGGAGACTTACTTTGGGGACTAGTTGGATG
GGAGGAGTACAGTGTTATTACTTTAACTACTTACTCACACTTCAAAATCGAACATACCGA
TGTTCCATTATCTTACTACACTGGACTTCTTGGTATAATATCTTATTCCTCTCTAATTAG
TTTGGTAGTGAAAAAAGCTTGGCTATTGGATGTTT

Last Updated on 06.12.2018 16:11