Sequences from confirmation amplicon


Confirmation sequences for line 031H11 and hit At3g59290 are shown.
Line ID 031H11
Locus At3g59290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21914647
Primers used for PCR 02vd - 8474, show primer details
   
Sequence Name 13-K117874-0022-031-H11C-02vd
GenBank Accession LN492236 [GenBank]
Sequencing primer gene specific primer, 02vd: 5'-TGATTCATTAACAAAATCAGCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117874-0022-031-H11C-02vd
AGCAGCTCCTGGAGGAGCAACTTGTGGGGTCTCTCCACCATCCCTTCAATGAAGAAATTT
TAAATGATTCATCATTTGAATTCAAAACTGCTAAGAAAAAAGTACTTCTCAATTTGCAGT
GAAGAGAATTGTCACCTTTCACTGTATACAGGGCTCCGTGATTCACTCACAGCTTCTTCA
TAACTAGGTGGAGCAGCACCAATATTTTGTTCAGAGAACTTTCTCTCTAGCCCCCCTCTA
TCAAATCTCAAAAGTAAGAGATAAGTATTGACATTTTTTCCATAATACTTAATACAAATA
ATAAAAAAGGGACCCTTCCAAAATCAGTACACGTCTCTGGAAATTACCTCCCATCTTGAG
AATGATCATCAGCTGGCGCATCATACAATTGATCATCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACCATTTATT
A
   
Sequence Name 01-K117873-0022-031-H11C-8409
GenBank Accession LN486764 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117873-0022-031-H11C-8409
CATTTCATTGAATATATCCTGATGATCAATTGTATGATGCGCCAGCTGATGATCATTCTC
AAGATGGGAGGTAATTTCCAGAGACGTGTACTGATTTTGGAAGGGTCCCTTTTTTATTAT
TTGTATTAAGTATTATGGAAAAAATGTCAATACTTATCTCTTACTTTTGAGATTTGATAG
AGGGGGGCTAGAGAGAAAGTTCTCTGAACAAAATATTGGTGCTGCTCCACCTAGTTATGA
AGAAGCTGTGAGTGAATCACGGAGCCCTGTATACAGTGAAAGGTGACAATTCTCTTCACT
GCAAATTGAGAAGTACTTTTTTCTTAGCAGTTTTGAATTCAAATGATGAATCATTTAAAA
TTTCTTCATTGAAGGGATGGTGGAGAGACCCCACAAGTTGCTCCTCCAGGAGCTGCTGCT
TCTCCTCTTGCTGAAAACATCAGCGTGGACAACAAAGCTGCTGATTTTGTAAAATGAATC

Last Updated on 06.12.2018 16:11