Sequences from confirmation amplicon


Confirmation sequences for line 032D02 and hit At3g44970 are shown.
Line ID 032D02
Locus At3g44970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16433774
Primers used for PCR CE01 - 8474, show primer details
   
Sequence Name 38-K105952-0022-032-D02C-CE01
GenBank Accession LN502985 [GenBank]
Sequencing primer gene specific primer, CE01: 5'-TTCTTTCCTTAGGAGTCACGGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K105952-0022-032-D02C-CE01
TATGTTAATGCAGGCATGCCGAGAGGGGATGCGAGAGATAAAGGATATATATACAATGAG
GAAAACGTCTGAGGAGAAGTACGATGACTTCCTCAACACGGCAATAGAAGAGTCAGAGAA
AGCAGGAGAGTTATTGAACGAAAATGCAATTATAACCCTCATCTTTACCCTTTCATGTGT
CACTCAAGATACCACCTCAAAGGCGATTTGCTTGGCTGTGAAATTCCTATTGGAAAACCC
AAAAGTTCTCGCAGAGTTAAAGGTAATATTTACATACAGATGTATAAGATGGTAAATTTT
ACATATAACCATGTGTTAGTATCGACCATGACATGTAGTAGTTTTTGCTAGTGATGTTGG
TGTGTCACAATATGAAAAAATTATGACACATATTATGGAGCAGCAATTGTAAATGGCTTC
ATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 54-K105953-0022-032-D02C-8409
GenBank Accession LN508483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K105953-0022-032-D02C-8409
CATTTACTGAAGCCATTTACAATTGCTGCTCCTAATATGTGTCATAATTTTTTCATATTG
TGACACACCAACATCACTAGCAAAAACTACTACATGTCATGGTCGATACTAACACATGGT
TATATGTAAAATTTACCATCTTATACATCTGTATGTAAATATTACCTTTAACTCTGCGAG
AACTTTTGGGTTTTCCAATAGGAATTTCACAGCCAAGCAAATCGCCTTTGAGGTGGTATC
TTGAGTGACACATGAAAGGGTAAAGATGAGGGTTATAATTGCATTTTCGTTCAATAACTC
TCCTGCTTTCTCTGACTCTTCTATTGCCGTGTTGAGGAAGTCATCGTACTTCTCCTCAGA
CGTTTTCCTCATTGTATATATATCCTTTATCTCTCGCATCCCCTCTCGGCATGCCTGCAT
TTAACATTAAGAGATCATGGATTCGTGTTAGTTTGATACTTACCGTGACTCCAGAGGGAA
AGA

Last Updated on 06.12.2018 16:11