Sequences from confirmation amplicon


Confirmation sequences for line 033B05 and hit At3g28510 are shown.
Line ID 033B05
Locus At3g28510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10686947
Primers used for PCR CW24 - 8474, show primer details
   
Sequence Name 35-K104113-0022-033-B05C-CW24
GenBank Accession LN501811 [GenBank]
Sequencing primer gene specific primer, CW24: 5'-ATTGAAGATATTGATTGCTCGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K104113-0022-033-B05C-CW24
GAGAGAAAAGAGGAGATGAAGAGGAGGATGGAGAGGAGAAGAAGGAAGGTGAGAAGAAGC
CCAAGGTAGATGATAAGCAGAGCAAGGTTACTTTGTCAGGTTTATTAAACTCCATTGATG
GGTTATGGTCAGCTTGTAGTGGTGAGAAGATCATAGTGTTCACAACTAATTTTGTGGATA
AGCTTGATCCAGCATTGATACGTAGAGGAAGAATGGACAACCACATTGAGATGTCTTATT
GCAAGTTTGAAGCATTCAAGGTTTTGGCTAAGAACTACTTGGAGATTGAAACACACGACT
TGTATGGAGAAATCGAAAGAAAGCTCGAAGAAACCGATATGTCCCCCGCGGATGTTGCGG
AGACTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 43-K104114-0022-033-B05C-8409
GenBank Accession LN504817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K104114-0022-033-B05C-8409
CACATCCGCGGGGGACTATCGGTTTCTTCGAGCTTTCTTTCGATTTCTCCATACAAGTCG
TGTGTTTCAATCTCCAAGTAGTTCTTAGCCAAAACCTTGAATGCTTCAAACTTGCAATAA
GACATCTCAATGTGGTTGTCCATTCTTCCTCTACGTATCAATGCTGGATCAAGCTTATCC
ACAAAATTAGTTGTGAACACTATGATCTTCTCACCACTACAAGCTGACCATAACCCATCA
ATGGAGTTTAATAAACCTGACAAAGTAACCTTGCTCTGCTTATCATCTACCTTGGGCTTC
TTCTCACCTTCCTTCTTCTCCTCTCCATCCTCCTCTTCATCTTCCTCTTTTTTCTTCTTT
CGCTGCCCCGTAAGATCAAGCGAGCAATCAATATTCTTCA
Primers used for PCR 61lf - 8474, show primer details
   
Sequence Name 79-K113840-0022-033-B05C-61lf
GenBank Accession LN513856 [GenBank]
Sequencing primer gene specific primer, 61lf: 5'-AAGTTGTTGTTGGATACAACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K113840-0022-033-B05C-61lf
TGATTGCTCGCTTGATCTTCGGGGCAGCGAAAGAAGAAAAAGAGGAAGATGAAGAGGAGG
ATGGAGAGGAGAAGAAGGAAGGTGAGAAGAAGCCCAAGGTAGATGATAAGCAGAGCAAGG
TTACTTTGTCAGGTTTATTAAACTCCATTGATGGGTTATGGTCAGCTTGTAGTGGTGAGA
AGATCATAGTGTTCACAACTAATTTTGTGGATAAGCTTGATCCAGCATTGATACGTAGAG
GAAGAATGGACAACCACATTGAGATGTCTTATTGCAAGTTTGAAGCATTCAAGGTTTTGG
CTAAGAACTACTTGGAGATTGAAACACACGACTTGTATGGAGAAATCGAAAGAAAGCTCG
AAGAAACCGATATGTCCCCCGCGGATGTTGCGGAGACTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
TATATCCGCAGCGTTATTA
   
Sequence Name 71-K113841-0022-033-B05C-8409
GenBank Accession LN512595 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K113841-0022-033-B05C-8409
GCGGGGGACTATCGGTTTCTTCGAGCTTTCTTTCGATTTCTCCATACAAGTCGTGTGTTT
CAATCTCCAAGTAGTTCTTAGCCAAAACCTTGAATGCTTCAAACTTGCAATAAGACATCT
CAATGTGGTTGTCCATTCTTCCTCTACGTATCAATGCTGGATCAAGCTTATCCACAAAAT
TAGTTGTGAACACTATGATCTTCTCACCACTACAAGCTGACCATAACCCATCAATGGAGT
TTAATAAACCTGACAAAGTAACCTTGCTCTGCTTATCATCTACCTTGGGCTTCTTCTCAC
CTTCCTTCTTCTCCTCTCCATCCTCCTCTTCATCTTCCTCTTTTTTCTTCTTTCGCTGCC
CCGTAAGATCAAGCGAGCAATCAATATCTTCAATCACAATGATAGACTTACTAGTTGTAT
CCACNAACAACT

Last Updated on 06.12.2018 16:11