Sequences from confirmation amplicon


Confirmation sequences for line 033B10 and hit At1g61950 are shown.
Line ID 033B10
Locus At1g61950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22901489
Primers used for PCR TR08 - 3144, show primer details
   
Sequence Name 10-K027636-022-033-B10C-TR08
GenBank Accession LN490507 [GenBank]
Sequencing primer gene specific primer, TR08: 5'-TACAATACCAATGATGGACCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K027636-022-033-B10C-TR08
TTATGGACAGTTTATTTATCAAATTACATTCGAAATTTTATGTACTGAACTAAAACTGTA
TTAAATCGGAGATTATGGTCCTTAATTCGGTTGAACCAATTTTGATTGGTGTGATTGTGA
GCAGCTCTTCATCATATTGCAAAACTCTTGATAATTTATACTTCCATCCTTTTTTAAAAA
CAAACAAAAATATGAATTAGAACATTTTAAAACTATCATTAAACTAAACAAATTGTGTTT
GGTATTTTATAAATTGAGATATTCGACTTACATTATCAGCATCAACTTCTGAGATAATCT
CTTTGATCATAATATCATCTCCCATATTATACTCTTTCATAGCTGTTTCTAATTCTTGTC
TCGAAATAAACCTACAAACACATGTAATTTGGTTATTAATCCGGTTTAATTCTGGTTCAT
CAGTTTGGTACATATCGGTTTCCGACACATACCCGCTGTTATCTTTATCGAAATGTTGAA
AAGCTTTGAACAAATTATCCTCTCTTTCTACTCTGAACCGGTTCATAGTTTAAACCGAAG
GCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCT
TATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTG
GAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACG
ATGCTCCTCG
   
Sequence Name 09-K027636-022-033-B10C-3144
GenBank Accession LN490033 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K027636-022-033-B10C-3144
CTATCCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGT
ACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATGAACCGGTTCAGAGTA
GAAAGAGAGGATAATTTGTTCAAAGCTTTTCAACATTTCGATAAAGATAACAGCGGGTAT
GTGTCGGAAACCGATATGTACCAAACTGATGAACCAGAATTAAACCGGATTAATAACCAA
ATTACATGTGTTTGTAGGTTTATTTCGAGACAAGAATTAGAAACAGCTATGAAAGAGTAT
AATATGGGAGATGATATTATGATCAAAGAGATTATCTCAGAAGTTGATGCTGATAATGTA
AGTCGAATATCTCAATTTATAAAATACCAAACACAATTTGTTTAGTTTAATGATAGTTTT
AAAATGTTCTAATTCATATTTTTGTTTGTTTTTAAAAAAGGATGGAAGTATAAATTATCA
AGAGTTTTGCAATATGATGAAGAGCTGCTCACAATCACACCAATCAAAATTGGTTCAACC
GAATTAAGGACCATAATCTCCGATTTAATTCATTTTTAGTTCAGTACATAAAATTTCGAA
TGTAATTTGGATAAATAAACTGTCCATAATATTCCTCATACAATTTGTTTTTGG
Primers used for PCR FI06 - 3144, show primer details
   
Sequence Name 59-K035347-022-033-B10C-FI06
GenBank Accession LN509922 [GenBank]
Sequencing primer gene specific primer, FI06: 5'-CTGATAAACCAATCGATAGTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K035347-022-033-B10C-FI6
GAATAAGCTTAAGAAGCTTGCATTTAAAGTAAGTATCTTTGATGTTTCGTTTTACTTGAT
GAAATTAGACCGGTTTTGATTCATTAATATGATTTGGTTTGGTTTAAGTTTATCGCGCAA
AATCTCAAAGAAGAGGAACTAAAGGGTCTCAAGACAATGTTCGCAAACATGGATACCGAT
AAGAGCGGTACAATCACTTACGACGAATTGAAATCCGGACTGGAAAAACTCGGTTCGAGG
TTAACCGAAACCGAAGTTAAGCAGCTCTTGGAAGATGTAAGGCGTTTTGTTCTCTTTACT
AACTTAAACTAACCGGTTTTGATCAATTTGGTTAATGTTTTTTGGTTTAGGCTGATGTCG
ATGGGAACGGTACTATTGATTACATTGAGTTCATTTCCCCGGGTACCCTGTCCTCTCCAA
ATGAAATGAACTTCCTAATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC
ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAAC
GTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCAATCTTTGG
   
Sequence Name 11-K035347-022-033-B10C-3144
GenBank Accession LN491019 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K035347-022-033-B10C-3144
CCATCCCCTATCCTTCGCAAGACCCTTCCTCTATATTAGGAAGTTCATTTCATTTGGAGA
GGACAGGGTACCCGGGGAAATGAACTCAATGTAATCAATAGTACCGTTCCCATCGACATC
AGCCTAAACCAAAAAACATTAACCAAATTGATCAAAACCGGTTAGTTTAAGTTAGTAAAG
AGAACAAAACGCCTTACATCTTCCAAGAGCTGCTTAACTTCGGTTTCGGTTAACCTCGAA
CCGAGTTTTTCCAGTCCGGATTTCAATTCGTCGTAAGTGATTGTACCGCTCTTATCGGTA
TCCATGTTTGCGAACATTGTCTTGAGACCCTTTAGTTCCTCTTCTTTGAGATTTTGCGCG
ATAAACTTAAACCAAACCAAATCATATTAATGAATCAAAACCGGTCTAATTTCATCAAGT
AAAACGAAACATCAAAGATACTTACTTTAAATGCAAGCTTCTTAAGCTTATTCATAGCTC
GGAGTTGCTTCATCCGCGACAAAACAGCACTATCGATGGGTTTTATCA

Last Updated on 06.12.2018 16:11