Sequences from confirmation amplicon


Confirmation sequences for line 033D05 and hit At1g11920 are shown.
Line ID 033D05
Locus At1g11920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4024311
Primers used for PCR CW69 - 8474, show primer details
   
Sequence Name 54-K104163-0022-033-D05C-CW69
GenBank Accession LN508462 [GenBank]
Sequencing primer gene specific primer, CW69: 5'-TTTCCATCTCTTCCAATCATATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K104163-0022-033-D05C-CW69
AGAATCATATATATGCATGTAAGATTTCATTGCATTATTGATGTGTGGTATTGTAATGAT
AGTTATTAAAATATTAACTATTTTAAATGAAGAGACCTGTTTTTTGCTAGGGTCATCAGA
AGCAACGAAGTAGTTGCCTTCACTAAAAATGGTTGGATCAGCACTTCCTCCGATCGCATA
CATTTGCCATTTCTCGTACCTATTGTTTGCTACATGCGCATATCCTCTTCGCACTCTGCC
ACGTCATCAGTCACATATCCATGTCAGCATTTTCTAATCTAGTTCAAGTAATCTAGATAT
CAATATGTATGCATGTACACTCACCTAGGCATTCTCTCAATAAGACCCGGTCCGAAATGG
TTGAACGCAATTGTAACTCTCATGATCTTATCTTCCACATTATTGTCATTATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 54-K104164-0022-033-D05C-8409
GenBank Accession LN508463 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K104164-0022-033-D05C-8409
GGACTGAGCCATAATGACATAATGTGGAAGATAAGATCATGAGAGTTACAATTGCGTTCA
ACCATTTCGGACCGGGTCTTATTGAGAGAATGCCTAGGTGAGTGTACATGCATACATATT
GATATCTAGATTACTTGAACTAGATTAGAAAATGCTGACATGGATATGTGACTGATGACG
TGGCAGAGTGCGAAGAGGATATGCGCATGTAGCAAACAATAGGTACGAGAAATGGCAAAT
GTATGCGATCGGAGGAAGTGCTGATCCAACCATTTTTAGTGAAGGCAACTACTTCGTTGC
TTCTGATGACCCTAGCAAAAAACAGGTCTCTTCATTTAAAATAGTTAATATTTTAATAAC
TATCATTACAATACCACACATCAATAATGCAATGAAATCTTACATGCATATATATGATTC
TATATATTTAGGTGACAAAAAGAATAGATAGTGGATATGATTGGAAGAGATGGAAA

Last Updated on 06.12.2018 16:11