Sequences from confirmation amplicon


Confirmation sequences for line 033D11 and hit At5g47610 are shown.
Line ID 033D11
Locus At5g47610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19301791
Primers used for PCR 11mm - 8474, show primer details
   
Sequence Name 63-K114263-0022-033-D11C-11mm
GenBank Accession LN511041 [GenBank]
Sequencing primer gene specific primer, 11mm: 5'-TAATTTGGGGTTTTGTTTCTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K114263-0022-033-D11C-11mm
AAGAGTACTTGAGTGATATAAAGAGACAAGAAACATGAAGTGTATTTTGTTCTTGTCTTT
CTCTCTCTTTTATCATCTAAATCCATATATTGACAAATTCAAACATGAAAACGAATTAAA
AAAAGAGCAATTTGCCTAGAATGTAGGCAACGTAGTGTGAGGACGACGTGTGGCAAACAT
GTGGATGATGATAAGCCACAGGACAAAGAAAGCAATCCCTCATCCATCGCAATAATATCC
ATTAATGTGAAGTGGACCAAAAGAGAGAGAAGCGAGTGTTACAGTCCTCAAAATAGAGAC
GAAACCATCCTAATCCTTCAAACTCTCTCACAGCTTCAAGACTCCACTGGAAGCGCCACT
ATTGTCCCTACACTCCTCTCCTTTATAAGGTCTCTTCTCACGAGGCTCTCTCTCAAACCA
TTAAAAGATGCGTTTGCTAGTAGCAGAAGCAGCTTCACCATTGTCCTCTGCTGCAACTCC
NACATGTAACTCTCATACTTGCAGATGGAAGCCTTACTCAAACTCTACTGACTTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAAA
   
Sequence Name 20-K114262-0022-033-D11C-8409
GenBank Accession LN495305 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114262-0022-033-D11C-8409
TTGAAGTCAGTAGAGTTTGAGTAAGGCTTCCATCTGCAAGTATGAGAGTTACATGTTGGA
GTTGCAGCAGAGGACAATGGTGAAGCTGCTTCTGCTACTAGCAAACGCATCTTTTAATGG
TTTGAGAGAGAGCCTCGTGAGAAGAGACCTTATAAAGGAGAGGAGTGTAGGGACAATAGT
GGCGCTTCCAGTGGAGTCTTGAAGCTGTGAGAGAGTTTGAAGGATTAGGATGGTTTCGTC
TCTATTTTGAGGACTGTAACACTCGCTTCTCTCTCTTTTGGTCCACTTCACATTAATGGA
TATTATTGCGATGGATGAGGGATTGCTTTCTTTGTCCTGTGGCTTATCATCATCCACATG
TTTGCCACACGTCGTCCTCACACTACGTTGCCTACATTCTAGGCAAATTGCTCTTTTTTT
AATTCGTTTTCATGTTTGAATTTGTCAATATATGGATTTAGATGATAAAAGAGAGAGAAA
GACAAGAACAAAATACACTTCATTGTTTCTTTGTCTCTTTTTATATCACTCAAGTTTACA
TCTTTTTTCTTTAACGATNAAAACAAAAGAAACAAAACCC

Last Updated on 06.12.2018 16:11