Sequences from confirmation amplicon


Confirmation sequences for line 033F02 and hit At4g18980 are shown.
Line ID 033F02
Locus At4g18980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10399293
Primers used for PCR 12mm - 8474, show primer details
   
Sequence Name 38-K114362-0022-033-F02C-12mm
GenBank Accession LN503067 [GenBank]
Sequencing primer gene specific primer, 12mm: 5'-CTTGGTTCAGTTCACAATAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K114362-0022-033-F02C-12mm
GCATATTGGCAATAGCAGATTCCTCCCATGAAAGTGGACCAAACGAACACTAAATTTACA
AGCGAAGAAACCAATTACTTCTCAAGCAAACCCCATAACTCAATTTATCATAAGATTTGA
TTGTTATGGGTTTTCTTTTTCTCAAGAAACCAAGCAATAATTTAAAAGAAAAATTTACAA
TTAAGTTAACCGGAAATTCAACATCTTACCAAATAAAATATGAGAAAATGTCCCCAATAT
TAAATTTTTCTTCACCAAACAATAAGAACCGTGATACATTGGTTCGTTTCAACGCTATGT
TATACGTGTAAAACATGATACACTAATTGGTTCGTTTCAAAGCTAAATCACATCCAAATT
TTGAAGAAAACCGACGTAAACCGAACTCAAATAGATCAAACTAAACTTGACCGGTTCTCA
TTTTATTTTGGGATCAATATTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 08-K114361-0022-033-F02C-8409
GenBank Accession LN489825 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114361-0022-033-F02C-8409
TTTATATTGATCCCAAAATAAAATGAGAACCGGTCAAGTTTAGTTTGATCTATTTGAGTT
CGGTTTACGTCGGTTTTCTTCAAAATTTGGATGTGATTTAGCTTTGAAACGAACCAATTA
GTGTATCATGTTTTACACGTATAACATAGCGTTGAAACGAACCAATGTATCACGGTTCTT
ATTGTTTGGTGAAGAAAAATTTAATATTGGGGACATTTTCTCATATTTTATTTGGTAAGA
TGTTGAATTTCCGGTTAACTTAATTGTAAATTTTTCTTTTAAATTATTGCTTGGTTTCTT
GAGAAAAAGAAAACCCATAACAATCAAATCTTATGATAAATTGAGTTATGGGGTTTGCTT
GAGAAGTAATTGGTTTCTTCGCTTGTAAATTTAGTGTTCGTTTGGTCCACTTTCATGGGA
GGAATCTGCTATTTGCCAATATGCTTGCTAATGTGAGCTGTATAAAAACTTTATTTGCTA
TTGTGACTGGAACCAA

Last Updated on 06.12.2018 16:11