Sequences from confirmation amplicon


Confirmation sequences for line 033F09 and hit At2g19810 are shown.
Line ID 033F09
Locus At2g19810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8551162
Primers used for PCR 17zg - 8474, show primer details
   
Sequence Name 33-K125614-0022-033-F09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K125614-0022-033-F09C-8409
TCATTGAATTATCCTGACGACAGGACTGAGTATGATGGTTTAATTGCTGAGAGACGCAAC
GACGTCGTTTCCCAGATTAGACCCGAGTGAACGACTCAGTAACGAACACGACGAGTCCGA
GTCACCTCGTGGACTCACCGGCGGCGAGTTAGACGACGGAGAAATCGAAAACTGAAACGC
TCTACGAATCGTAGGAGACAAAACGTCGAACGAATCAACACGATCAGGGCTTTGATTAGG
CAAAACCCTAATCTGATCCGGCGAATGAGCAAAGAAACAAACACGACGGCGACAGTTACC
ACCGTCTTTACACGGCTGAGTCCGGTAACGCGCCGGGTGAAGCCAACACTCAAAAACACC
ATGAGAAAACTCACATGCGTCTCCTCTCTTGCAACAACCTTTACGAAACTCAGGACACGC
CGTACCGGAGTAATGAAACTTCCTCGGATCACGACGGCGAGCTTTTTCTCCGGGATGAGC
GTAAGGACACTCCGTCCAATCATGGCTCCGGCCACGAGCACAACGTCTCACTTTAAACTC
ATACATCCGAAAATGATCGCATGTATAAGCATCGATTGGTGAATCCGGACCCGAGAGATC
CGGATCTGAATCCGGATCCGGTTCGTTTGACGGAAGATAACGTTGTAACTCAGCCAAAGC
TTCCATCATACTACTTCCTCCGTCTGGACTTCCGTAAATATCAGACACCGTTAGATCTTC
AGAAAGTGGCCATGGAGGAATATGAACCGTTGGATTAAAACCACGATGAGATTCTCCGAT
CATCATTTTTTTACAACAATTCACTCAAGAAGAAAAAATCACATTCGTCTGAGTTGGTTT
TGTTTTTTGTGTTTGTGTTTGCTAAATCTCCGGCAAAGCTTTACTTCTTTATAAACTGAT
GCTGAGTAGTGGGATATACTAAAACCACCTTTGGGTTA
   
Sequence Name 10-K125613-0022-033-F09C-17zg
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 17zg: 5'-AGTAATATAACAGCCGCTCACTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K125613-0022-033-F09C-17zg
AATCATCCACGTCAATTAATATCCTTGTGGAAAGGTCTCAAGCCGCTGTCCTTAACCTCT
GCATCACACACACGTCACTGTAGCTTCTCACTTTCGATTTTAACCGCGGTTGATAAAAGC
AGAAATATTTAACCATGGTTAGGTTTTAACCCAAAGGTGGTTTAAGTATATCCCACTACT
CAGCATCAGTTTATAAAGAAGTAAAGCTTTGCCGGAGATTTAGCAAACACAAACACAAAA
AACAAAACCAACTCAGACGAATGTGATTTTTTCTTCTTGAGTGAATTGTTGTAAAAAAAT
GATGATCGGAGAATCTCATCGTGGTTTTAATCCAACGGTTCATATTCCTCCATGGCCACT
TTCTGAAGATCTAACGGTGTCTGATATTTACGGAAGTCCAGACGGAGGAAGTAGTATGAT
GGAAGCTTTGGCTGAGTTACAACGTTATCTTCCGTCAAACGAACCGGATCCGGATTCAGA
TCCGGATCTCTCGGGTCCGGATTCACCAATCGATGCTTATACATGCGATCATTTTCGGAT
GTATGAGTTTAAAGTGAGACGTTGTGCTCGTGGCCGGAGCCATGATTGGACGGAGTGTCC
TTACGCTCATCCCGGAGAAAAAGCTCGCCGTCGTGATCCGAGGAAGTTTCATTACTCCGG
TACGGCGTGTCCTGAGTTTCGTAAAGGTTGTTGCAAGAGAGGAGACGCATGTGAGTTTTC
TCATGGTGTTTTTGAGTGTTGGCTTCACCCGGCGCGTTACCGGACTCAGCCGTGTAAAGA
CGGTGGTAACTGTCGCCGTCGTGTTTGTTTCTTTGCTCATTCGCCGGATCAGATTAGGGT
TTTGCCTAATCAAAGCCCTGATCGTGTTGATTCGTTCGACGTTTTGTCTCCTACGATTCG
TAGAGCGTTTCAGTTTT

Last Updated on 06.12.2018 16:11