Sequences from confirmation amplicon


Confirmation sequences for line 033G12 and hit At2g25310 are shown.
Line ID 033G12
Locus At2g25310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10778820
Primers used for PCR 02kh - 8474, show primer details
   
Sequence Name 67-K113549-0022-033-G12C-02kh
GenBank Accession LN511855 [GenBank]
Sequencing primer gene specific primer, 02kh: 5'-ATTTTGGGGTGAAAATGTAGACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113549-0022-033-G12C-02kh
ATTTAGCAACGAAACTAGTATATAGAATTGTAGATTCCNGGTTTAGAGACGAAAAAAAAA
AAATCCGGTTTTAAAACATCGACCAAATAGGGTTAAGTTAACTGAACTTTTCAGGGTAAA
AAAAGTAAAATAAAATAAAATTAAACCAGTACCTTCTCATTTATCCAAAAGGTTTTGATT
TGGTTTTCCTTAACATCCTTCGTCGCCGGAATTTGACAACAAAAATCAAACTTCATCAGC
TTACGTCAATCTACTTACAACGGCAATAACAATAACTTCAATAATCAAATCCAGTCCTAC
TCTCGCTTTCATCGTCTTACAAATTTGCTTCATCTTCTTTGCTTCCACTTTCCCTTGCTC
TTCTGGGTAAGTAAATCGTTCTCTGTCTTTAATTTCTCTGCTAAATCATCTCAATTATCT
AGTCAATGCAATTTCCGGCAAATTTCTCCTGTTGATAATTTGTCTCAATTTGATAATTTA
TTTGCTTGCCAAGTTCAGTACTTTGATTTTTTGGAAATCCAAAAATTTATTTCTCTTTGT
GGGGTTTATTGGGCAGGTCTAAGGATAGTTACACCATTACTCTACCAGTAAATGGATCAC
CAATGAGTATAATGGTCAATATGGAAAAAAAAAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAAATTTC
   
Sequence Name 02-K113548w-0022-033-G12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K113548w-0022-033-G12C-8409
TACTATCCTCGACCTGCACATAAACCACACAAAGAGAAATAAATTCTCGGATCTCCAAAA
AATCAAAGTACTGAACTCGGCAAGCAACTAAATCATCAAATTGAGACAAATTATCAACAG
AAGAAATTCGCAGGAAATTGCATCGACTAGATAATTGAGATGATTTAGCAGAGAAATCAA
AGACAGAGAACGATTTACTTACCCAGAAGAGCAAGGGAAAGTGGAAGCAAAGAAGATGAA
GCAAATCTGTAAGACGATGAAAGCGAGAGTAGGACTGGATCTGATGATCGACGTCATCGT
CATAGCCGTCGTACGTAGATCGACGTAAGCTGATGAAGTCTGATATTAGTCGTCAAATTC
CGGCGACGAAGGATGTTAAGGAAAACCAAATCAAAACCTTCTGGATAACTGAGAAGGTAC
TGGTTTAATTTTATTTTATTTTACTTTTTTTACCCTGAAAAGTTCAGTTAACTTAACCCT
ATTTGGTCGATGTTCTAAACCGGATTTTTTTTTTTTCGTCTCTAAACCGGAATCTACAAT
TCTATATACTTAGTTTCGTTGGCTAAAAGGCGATCCAATTTGCACTTAGACGGTCTACAT
TTTCACCCCAA

Last Updated on 06.12.2018 16:11