Sequences from confirmation amplicon


Confirmation sequences for line 034B03 and hit At2g06166 are shown.
Line ID 034B03
Locus At2g06166 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2411172
Primers used for PCR RM10 - 8474, show primer details
   
Sequence Name 01-K026133-0022-034-B03C-8409
GenBank Accession LN486427 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K026133-0022-034-B03C-8409
CATATCAAATTATAAGAAAGTATTTAGACTCATTTTTGGAGAGTAAGGAGCTTTATAAAT
CATTGCCCACACACACAAGTGGAGAAATCAGCTAAGATATTCATTGGTGTTCTACTACTT
TCAACGTGTGAGTTTCAAGACAAATTTTATGTTCCAATTGTATTCCATATTCATCCTTCT
CATTATATCATGAGTGTGAATATATAAATCAATTATATGGTTTTTAAAGGTACATCCATT
ATGGTACGAATCAGATATGTATCTTGCAAGACTAACTTTGATTGTGTTAACCTCAAATGT
CCAACGCCATTTGTAACCCCCAAATGTGTGAGCGGTGGTTGTGAATGCCCCCTTAAAGAA
CTCTTGACATTACTGAGTGATACCAATTATGGTGTGGCTGCTTGCATTGACTATTGCAAG
GCAAAAGGTGAAAATGCTTATACATGTATCTTGAACCATTGTTATTGTCGCAAACCTTCC
   
Sequence Name 02-K026133-0022-034-B03C-RM10
GenBank Accession LN486883 [GenBank]
Sequencing primer gene specific primer, RM10: 5'-AATATTACATGGAAGGTTTGCGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K026133-0022-034-B03C-RM10
ATGGTTCAAGATACATGTATAAGCATTTTCACCTTTTGCCTTGCAATAGTCAATGCAAGC
AGCCACACCATAATTGGTATCACTCAGTAATGTCAAGAGTTCTTTAAGGGGGCATTCACA
ACCACCGCTCACACATTTGGGGGTTACAAATGGCGTTGGACATTTGAGGTTAACACAATC
AAAGTTAGTCTTGCAAGATACATATCTGATTCGTACCATAATGGATGTACCTTTAAAAAC
CATATAATTGATTTATATATTCACACTCATGATATAATGAGAAGGATGAATATGGAATAC
AATTGGAACATAAAATTTGTCTTGAAACTCACACGTTGAAAGTAGTAGAACACCAATGAA
TATCTTAGCTGATTTCTCCACTTGTGTGTGTGGGCAATGATTTATAAAGCTCCTTACTCT
CCAAAAATGAGTCTAAATACTTTCTTATAATCTGATATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11