Sequences from confirmation amplicon


Confirmation sequences for line 034C05 and hit At5g47840 are shown.
Line ID 034C05
Locus At5g47840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19375588
Primers used for PCR RZ03 - 3144, show primer details
   
Sequence Name 15-K016840-022-034-C05C-3144
GenBank Accession LN492744 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K016840-022-034-C05C-3144
GGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAA
ACTATCTGGGGTGACGGAGAGGCACGATGCCGGTATAAAGTCACCGGCGGCGGAGAAATA
GAATTCACACAGCCCGTCATGTCTCTCTCCCACTCTCTTTCTGTCAATGAGCCTGTGGTG
GTGAATACAGAGGGACCAGAGGACTTCACTGTTCCGATAATTTAGTGGAGGGCCCAAACC
TAAATGGACTCACTTTAATTATTTTAAGGGTCTATTTGTTTGATCATTTGGTATACTTGA
ATTGTTTTAAGGGCCTATTCGTTTGCATTTGGTAT
   
Sequence Name 16-K016840-022-034-C05C-RZ03
GenBank Accession LN493210 [GenBank]
Sequencing primer gene specific primer, RZ03: 5'-AAATACCAAATGCAAACGAATAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K016840-022-034-C05C-RZ03
TTAAAACAATTCAAGTATACCAAATGATCAAACAAATAGACCCTTAAAATAATTAAAGTG
AGTCCATTTAGGTTTGGGCCCTCCACTAAATTATCGGAACAGTGAAGTCCTCTGGTCCCT
CTGTATTCACCACCACAGGCTCATTGACAGAAAGAGAGTGGGAGAGAGACATGACGGGCT
GTGTGAATTCTATTTCTCCGCCGCCGGTGACTTTATACCGGCATCGTGCCTCTCCGTCAC
CCCAGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGT
GCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTT
GGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG
Primers used for PCR FI07 - 8474, show primer details
   
Sequence Name 16-K028095-022-034-C05C-8409
GenBank Accession LN493295 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K028095-022-034-C05C-8409
CCATTTACAATTGACGGGTACACTCTCTTTACCGGCACCGACGTGTATCAAGATCTCCGT
CGATTATCGCTCCCAAATTTCAGGTGAATGATTGTTTTGACCTCCGATTTTTGAATGCGA
TTTGCGAGCTTGTAATTTTGGATTTTATTGTGTGTGGAAGGAATATAGATTGTGGCGGCT
GAAAAATCGGAGCCTCTGAAAATTATGATATCAGGAGCTCCTGCTTCTGGTAAAGGTACA
CAATGCGAGCTGATTACTCACAAAGTAAGCTTATTGATCTTGTTATTGGATACTATTCTC
ATGAACCCAATTCAATTTGAAAATACTTTGATCGATTTTGTCAATGAACCTTAACTCGGC
CACTTATAGCATTGCATGAGCTGATTCAGGGGGTTCTCTAGTAATGATATCAAGCATTAA
ACCATTTTTTTGGCTGTATTTTGTGATGATGACTACGTTTTAGTGCTCTTTTATGAAGAG
GTTAACATGCTAAAGTAAAGCTTAGGTTGTTGAATAAGTAAGGAAAATCGTGCTCTTAAT
TTAAAACAAAAACCCGTGTTTCTGCAGAACTTGCTACTCATTTTTACGGATAATTTTTAT
GTTTCTGTTTTCATGGAAGACGTCAAGCT
   
Sequence Name 16-K028095-022-034-C05C-FI07
GenBank Accession LN493296 [GenBank]
Sequencing primer gene specific primer, FI07: 5'-CTAAACCAGAAAGCTTGACGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K028095-022-034-C05C-FI07
TATCCGTAAAATGAGTAGCAAGTTCTGCAGAAACACGGGTTTTTGTTTTAAATTAAGAGC
ACGATTTTCCTTACTTATTCAACAACCTAAGCTTTACTTTAGCATGTTAACCCTCTTCAT
AAAAGAGCACTAAAACGTAGTCATCATCACAAAATACAGCCAAAAAAATGGTTTAATGCT
TGATATCATTACTAGAGAACCCCCTGAATCAGCTCATGCAATGCTATAAGTGGCCGAGTT
AAGGTTCATTGACAAAATCGATCAAAGTATTTTCAAATTGAATTGGGTTCATGAGAATAG
TATCCAATAACAAGATCAATAAGCTTACTTTGTGAGTAATCAGCTCGCATTGTGTACCTT
TACCAGAAGCAGGAGCTCCTGATATCATAATTTTCAGAGGCTCCGATTTTTCAGCCGCCA
CAATCTATATTCCTTCCACACACAATAAAATCCAAAATTACAAGCTCGCAAATCGCATTC
AAAAATCGGAGGTCAAAACAATCATTCACCTGAAATTTGGGAGCGATAATCGACGGAGAT
CTTGATACACGTCGGTGCCGGTAAAGAGAGTGTAACCCGTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACGAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11