Sequences from confirmation amplicon


Confirmation sequences for line 034D08 and hit At1g22015 are shown.
Line ID 034D08
Locus At1g22015 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7752454
Primers used for PCR JA18 - 8474, show primer details
   
Sequence Name 27-K100938-0022-034-D08C-8409
GenBank Accession LN498304 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K100938-0022-034-D08C-8409
ATGAATGCAATGTAGTCTAATGTGTGTTTTTTGTTTAGTAGACTAAGATTCTTACTTTAA
TGTGTTGGCAGTTCAACGCCAAACAGTATGTTGGATAAGGAGATTGATTCAGAAGATGCT
CAATACAACGATTTCTTTAGGCTGGTTAGACCACTACAAACTTGATTCTTCTAGGATTTG
GTTTACGGTTTTCTTGATTATTTTTTAACTCGTTGCAGGATCATGTCGAAGGATATTACA
ATCTATCTGCAAAAACCAAAAGCTTCTTCTCTAGTGCAGTTGCAAAGTGGGATGCTGAGT
TTTATGTCAAGATTGATGATGATGTTCACGTCAATCTTGGTACCAAAATATGTTTCTAGC
ATAAAAAAAATTATAATATTTTTTCCTTGAACTAAAATTGTTGAATATATTTGCTCACCT
CTTTGCTTTTTCAGGCACGCTTGCTTCCACATTAGCA
   
Sequence Name 27-K100944-0022-034-D08C-JA18
GenBank Accession LN498305 [GenBank]
Sequencing primer gene specific primer, JA18: 5'-TTAGAACGGTGACTTGCTAATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K100944-0022-034-D08C-JA18
TGAGCAATATATTCACAATTTTAGTTCAAGGAAAAAATATTATAATTTTTTTTATGCTAG
AAACATATTTTGGTACCAAGATTGACGTGAACATCATCATCAATCTTGACATAAAACTCA
GCATCCCACTTTGCAACTGCACTAGAGAAGAAGCTTTTGGTTTTTGCAGATAGATTGTAA
TATCCTTCGACATGATCCTGCAACGAGTTAAAAAATAATCAAGAAAACCGTAAACCAAAT
CCTAGAAGAATCAAGTTTGTAGTGGTCTAACCAGCCTAAAGAAATCGTTGTATTGAGCAT
CTTCTGAATCAATCTCCTTATCCAACATACTGTTTGGCGTTGAACTGCCAACACATTAAA
GTAAGAATCTTAGTCTACTTAAACAAAAAACACACATTAGACTACCTTGCAATCATTCAC
CTGTGTCCCGGAAATCTACATGGATTAGCCATGAATATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCCATTCAAAAATGT

Last Updated on 06.12.2018 16:11